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TdT Antibody (DNTT/4506R) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08564

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # DNTT/4506R

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Summary for TdT Antibody (DNTT/4506R) - Azide and BSA Free

Immunogen

Synthetic peptide corresponding to residues within aa1-100 of TdT was used as an immunogen. (Uniprot: P04053)

Localization

Nucleus.

Marker

Acute Lymphoblastic Leukemia Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Theoretical MW

58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07684).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for TdT Antibody (DNTT/4506R) - Azide and BSA Free

Immunohistochemistry-Paraffin: TdT Antibody (DNTT/4506R) - Azide and BSA Free [NBP3-08564]

Immunohistochemistry-Paraffin: TdT Antibody (DNTT/4506R) - Azide and BSA Free [NBP3-08564]

Immunohistochemistry-Paraffin: TdT Antibody (DNTT/4506R) - Azide and BSA Free [NBP3-08564] - IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using TdT antibody (DNTT/4506R) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 : HRP-polymer, 30min. DAB, 5min.
Immunohistochemistry-Paraffin: TdT Antibody (DNTT/4506R) - Azide and BSA Free [NBP3-08564]

Immunohistochemistry-Paraffin: TdT Antibody (DNTT/4506R) - Azide and BSA Free [NBP3-08564]

Immunohistochemistry-Paraffin: TdT Antibody (DNTT/4506R) - Azide and BSA Free [NBP3-08564] - Formalin-fixed, paraffin-embedded human thymus stained with TdT Recombinant Rabbit Monoclonal Antibody (DNTT/4506R).
Immunohistochemistry-Paraffin: TdT Antibody (DNTT/4506R) - Azide and BSA Free [NBP3-08564]

Immunohistochemistry-Paraffin: TdT Antibody (DNTT/4506R) - Azide and BSA Free [NBP3-08564]

Immunohistochemistry-Paraffin: TdT Antibody (DNTT/4506R) - Azide and BSA Free [NBP3-08564] - Formalin-fixed, paraffin-embedded human thymus stained with TdT Recombinant Rabbit Monoclonal Antibody (DNTT/4506R).

Applications for TdT Antibody (DNTT/4506R) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: TdT

TdT is a nuclear enzyme involved in the recombination of Immunoglobulin and T cell receptor genes. TdT is expressed in early lymphoid cells and by tumor cells in certain classes of acute lymphoid leukemia.

Long Name

Terminal Deoxynucleotidyl Transferase

Alternate Names

DNTT

Gene Symbol

DNTT

Additional TdT Products

Product Documents for TdT Antibody (DNTT/4506R) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TdT Antibody (DNTT/4506R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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