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TdT Antibody (PD01-00)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33003

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-33003

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PD01-00

Concentration

1 mg/ml

Product Summary for TdT Antibody (PD01-00)

Immunogen

Synthetic peptide. (Uniprot: P04053)

Localization

Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TdT Antibody (PD01-00)

TdT Antibody (PD01-00)

Immunohistochemistry: TdT Antibody (PD01-00) [NBP3-33003]

Immunohistochemistry: TdT Antibody (PD01-00) [NBP3-33003] - Immunohistochemical analysis of paraffin-embedded human thymus tissue with Rabbit anti-TdT antibody (NBP3-33003) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33003) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications for TdT Antibody (PD01-00)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TdT

TdT is a nuclear enzyme involved in the recombination of Immunoglobulin and T cell receptor genes. TdT is expressed in early lymphoid cells and by tumor cells in certain classes of acute lymphoid leukemia.

Long Name

Terminal Deoxynucleotidyl Transferase

Alternate Names

DNTT

Gene Symbol

DNTT

Additional TdT Products

Product Documents for TdT Antibody (PD01-00)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TdT Antibody (PD01-00)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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