TdT Antibody (TDT/1393)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07673

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # TDT/1393

Concentration

0.2 mg/ml

View all TdT Antibodies »

Product Summary for TdT Antibody (TDT/1393)

Immunogen

Recombinant fragment (around aa 52-192) of human TdT protein (exact sequence is proprietary) (Uniprot: P04053)

Localization

Nucleus.

Marker

Acute Lymphoblastic Leukemia Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08484)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for TdT Antibody (TDT/1393)

Immunohistochemistry-Paraffin: TdT Antibody (TDT/1393) [NBP3-07673]

Immunohistochemistry-Paraffin: TdT Antibody (TDT/1393) [NBP3-07673]

Immunohistochemistry-Paraffin: TdT Antibody (TDT/1393) [NBP3-07673] - Formalin-fixed, paraffin-embedded human thymus stained with TdT Antibody (TDT/1393).
Immunohistochemistry-Paraffin: TdT Antibody (TDT/1393) [NBP3-07673]

Immunohistochemistry-Paraffin: TdT Antibody (TDT/1393) [NBP3-07673]

Immunohistochemistry-Paraffin: TdT Antibody (TDT/1393) [NBP3-07673] - Formalin-fixed, paraffin-embedded human Thymus stained with TdT Mouse Monoclonal Antibody (TDT/1393).
Immunohistochemistry-Paraffin: TdT Antibody (TDT/1393) [NBP3-07673]

Immunohistochemistry-Paraffin: TdT Antibody (TDT/1393) [NBP3-07673]

Immunohistochemistry-Paraffin: TdT Antibody (TDT/1393) [NBP3-07673] - Formalin-fixed, paraffin-embedded human thymoma stained with TdT Antibody (TDT/1393).
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Applications for TdT Antibody (TDT/1393)

Application
Recommended Usage

ELISA

2-4 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: TdT

TdT is a nuclear enzyme involved in the recombination of Immunoglobulin and T cell receptor genes. TdT is expressed in early lymphoid cells and by tumor cells in certain classes of acute lymphoid leukemia.

Long Name

Terminal Deoxynucleotidyl Transferase

Alternate Names

DNTT

Gene Symbol

DNTT

UniProt

P04053 (Human)

Additional TdT Products

Product Documents for TdT Antibody (TDT/1393)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TdT Antibody (TDT/1393)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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