TEAD4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32765
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human TEAD4. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (86%).
Localization
Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for TEAD4 Antibody
![Immunohistochemistry-Paraffin: TEAD4 Antibody [NBP1-32765]](https://resources.bio-techne.com/images/products/TEAD4-Antibody-Immunohistochemistry-Paraffin-NBP1-32765-img0005.jpg "Immunohistochemistry-Paraffin: TEAD4 Antibody [NBP1-32765]")
Immunohistochemistry-Paraffin: TEAD4 Antibody [NBP1-32765]
Immunohistochemistry-Paraffin: TEAD4 Antibody [NBP1-32765] - SW480 xenograft, using TEAD4 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: TEAD4 Antibody [NBP1-32765] -
Western Blot: TEAD4 Antibody [NBP1-32765] - HepG2 whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TEAD4 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunocytochemistry/ Immunofluorescence: TEAD4 Antibody [NBP1-32765] -
Immunocytochemistry/ Immunofluorescence: TEAD4 Antibody [NBP1-32765] - TEAD4 antibody detects TEAD4 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TEAD4 stained by TEAD4 antibody (NBP1-32765) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar= 10 um.Applications for TEAD4 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Reviewed Applications
Read 1 review rated 5 using NBP1-32765 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: TEAD4
Alternate Names
EFTR-2, hRTEF-1B, related transcription enhancer factor 1B, RTEF1RTEF-1, TCF13L1MGC9014, TEA domain family member 4TEF3, TEAD-4, TEF-3, TEFR-1, Transcription factor 13-like 1, Transcription factor RTEF-1, transcriptional enhancer factor 1-related, transcriptional enhancer factor 3, transcriptional enhancer factor TEF-3
Gene Symbol
TEAD4
Additional TEAD4 Products
Product Specific Notices for TEAD4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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