Tenascin C Antibody (SPM319) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47920
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Rat (Negative)
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM319
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Human breast carcinoma (Uniprot: P24821)
Reactivity Notes
Does not react with Rat.
Localization
Connective tissue matrix
Specificity
In Western blotting, it reacts with two bands of ~MW of 210kDa and 300kDa, identified as two isoforms of Tenascin C. Specificity of this monoclonal antibody is validated by sequential immunoprecipitation with a PAb against Tenascin C. Tenascin C is a multifunctional, disulfide-linkedhexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neo-vasculature and stroma of undifferentiated tumors. In adults, it is restricted to certain epithelial-stromal interfaces and increases markedly in hyper-proliferative diseases and in stroma of many neoplasms, including gliomas, breast, squamous and lung carcinomas.
Marker
Stromal Marker For Epithelial Malignancy
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44583).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Tenascin C Antibody (SPM319) - Azide and BSA Free
Immunohistochemistry-Paraffin: Tenascin C Antibody (SPM319) - Azide and BSA Free [NBP2-47920]
Immunohistochemistry-Paraffin: Tenascin C Antibody (SPM319) - Azide and BSA Free [NBP2-47920] - Human lung carcinoma stained with Tenascin C monoclonal antibody (SPM319) at 4ug/ml. Antigen retrieval in 10mM Tris with 1mM EDTA, pH 9.0; ABC detection system with DAB Chromogen. Note staining of connective tissue.Applications for Tenascin C Antibody (SPM319) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells in 0.1 ml
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
0.5 - 1.0 ug/ml
Immunohistochemistry-Paraffin
2 - 4 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Tenascin C
Alternate Names
Cytotactin, HXB, Tenascin J1, TNC
Gene Symbol
TNC
Additional Tenascin C Products
Product Documents for Tenascin C Antibody (SPM319) - Azide and BSA Free
Product Specific Notices for Tenascin C Antibody (SPM319) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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