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Teneurin-1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-41315

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Antibody was raised against an 18 amino acid peptide near the amino terminus of human TENM1. The immunogen is located within the first 50 amino acids of TENM1. Amino Acid Squence: SDESEDGRKPRQSFNSRE

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken: (83%)

Specificity

TENM1 antibody is predicted to not cross-react with other members of the TENM family.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

280 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Teneurin-1 Antibody - BSA Free

Western Blot: Teneurin-1 AntibodyBSA Free [NBP2-41315]

Western Blot: Teneurin-1 AntibodyBSA Free [NBP2-41315]

Western Blot: Teneurin-1 Antibody [NBP2-41315] - Western blot analysis of TENM1 in human brain tissue lysate with TENM1 antibody at 1 ug/ml.
Teneurin-1 Antibody - BSA Free

Immunoprecipitation: Teneurin-1 Antibody - BSA Free [NBP2-41315] -

Immunoprecipitation: Teneurin-1 Antibody - BSA Free [NBP2-41315] - HINT1 exhibits isopeptidase activity. (A) HINT1 interactions with the proteins used in the study. (I) HINT1 but not its paralogs forms stable complexes with RGSZ2 proteins. (II) ICD teneurin1 interacts with HINT1 but not with GST. (III, IV) HINT1 associations with RanGAP1 & SP100 were weak or not detected. (B) HINT1 exhibits SUMO protease activity on sumoylated RanGAP1, which is regulated by calcium & CaM. (I) HINT1 (2 μM) but not SENP2 (0.3 μM) requires calcium-activated CaM (6 μM) to exhibit sumoylase activity. (II) Removal of 10 mM MgCl2 from the sumoylation buffer greatly improves Ca2+-CaM-dependent HINT1 isopeptidase activity on sumoylated RanGAP1. (III, IV) HINT1 exhibits isopeptidase activity on sumoylated RanGAP1 in the presence of increasing concentrations of CaCl2 & of CaM. Details of immunoblot detection in “Materials & Methods” section & Supplementary Figures S5 & S6. GST, glutathione S-transferase; ICD, intracellular domain; RanGAP1, Ran GTPase-activating protein 1; RGSZ2, regulator of G protein signaling 17 (Z2); SENP, sentrin-specific protease. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31088288), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Teneurin-1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Teneurin-1 Antibody - BSA Free [NBP2-41315] -

Immunocytochemistry/ Immunofluorescence: Teneurin-1 Antibody - BSA Free [NBP2-41315] - Immunofluorescence of Teneurin-1 in mouse brain tissue with Teneurin-1 antibody at 20 u/mL.

Applications for Teneurin-1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

5 ug/mL

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1 - 2 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Teneurin-1

Teneurin-1, also known as Teneurin Transmembrane Protein 1 or Ten-1, contains a 305 kDa and a 306 kDa isoform, and is involved in regulating the connections within the nervous system and neural development. Teneurin-1 is not currently being used in any disease research. The protein has not been found to interact with any other proteins, but is linked to transcription, stress and immune responses, and signal transduction.

Alternate Names

ODZ1, ODZ3, Ten-1, TEN-M1, Tenascin-M1, Teneurin1, TNM

Gene Symbol

TENM1

Additional Teneurin-1 Products

Product Documents for Teneurin-1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Teneurin-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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