Teneurin-1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-41315
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Antibody was raised against an 18 amino acid peptide near the amino terminus of human TENM1. The immunogen is located within the first 50 amino acids of TENM1. Amino Acid Squence: SDESEDGRKPRQSFNSRE
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Chicken: (83%)
Specificity
TENM1 antibody is predicted to not cross-react with other members of the TENM family.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
280 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Teneurin-1 Antibody - BSA Free
Western Blot: Teneurin-1 AntibodyBSA Free [NBP2-41315]
Western Blot: Teneurin-1 Antibody [NBP2-41315] - Western blot analysis of TENM1 in human brain tissue lysate with TENM1 antibody at 1 ug/ml.Immunoprecipitation: Teneurin-1 Antibody - BSA Free [NBP2-41315] -
Immunoprecipitation: Teneurin-1 Antibody - BSA Free [NBP2-41315] - HINT1 exhibits isopeptidase activity. (A) HINT1 interactions with the proteins used in the study. (I) HINT1 but not its paralogs forms stable complexes with RGSZ2 proteins. (II) ICD teneurin1 interacts with HINT1 but not with GST. (III, IV) HINT1 associations with RanGAP1 & SP100 were weak or not detected. (B) HINT1 exhibits SUMO protease activity on sumoylated RanGAP1, which is regulated by calcium & CaM. (I) HINT1 (2 μM) but not SENP2 (0.3 μM) requires calcium-activated CaM (6 μM) to exhibit sumoylase activity. (II) Removal of 10 mM MgCl2 from the sumoylation buffer greatly improves Ca2+-CaM-dependent HINT1 isopeptidase activity on sumoylated RanGAP1. (III, IV) HINT1 exhibits isopeptidase activity on sumoylated RanGAP1 in the presence of increasing concentrations of CaCl2 & of CaM. Details of immunoblot detection in “Materials & Methods” section & Supplementary Figures S5 & S6. GST, glutathione S-transferase; ICD, intracellular domain; RanGAP1, Ran GTPase-activating protein 1; RGSZ2, regulator of G protein signaling 17 (Z2); SENP, sentrin-specific protease. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31088288), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Immunocytochemistry/ Immunofluorescence: Teneurin-1 Antibody - BSA Free [NBP2-41315] -
Immunocytochemistry/ Immunofluorescence: Teneurin-1 Antibody - BSA Free [NBP2-41315] - Immunofluorescence of Teneurin-1 in mouse brain tissue with Teneurin-1 antibody at 20 u/mL.Applications for Teneurin-1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
20 ug/mL
Immunohistochemistry
5 ug/mL
Immunohistochemistry-Paraffin
5 ug/ml
Western Blot
1 - 2 ug/mL
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Teneurin-1
Alternate Names
ODZ1, ODZ3, Ten-1, TEN-M1, Tenascin-M1, Teneurin1, TNM
Gene Symbol
TENM1
Additional Teneurin-1 Products
Product Documents for Teneurin-1 Antibody - BSA Free
Product Specific Notices for Teneurin-1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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