TERT Antibody (2C4)

Western Blot: TERT Antibody (2C4) [NB100-317]

Western Blot: TERT Antibody (2C4) [NB100-317] - 1:500 dilution on MJ90 cells.

Immunohistochemistry-Paraffin: TERT Antibody (2C4) [NB100-317]

Immunohistochemistry-Paraffin: TERT Antibody (2C4) [NB100-317] - Normal human pancreas showing moderate staining of exocrine cells and a subset of islets of Langerhans.

Flow Cytometry: TERT Antibody (2C4) [NB100-317]

Flow Cytometry: TERT Antibody (2C4) [NB100-317] - An intracellular stain was performed on Jurkat cells with TERT Antibody (2C4) NB100-317 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse IgM Alexa Fluor 488-conjugated secondary antibody.

Western Blot: TERT Antibody (2C4) [NB100-317] -

Western Blot: TERT Antibody (2C4) [NB100-317] - The interaction of TERT with p65 in murine macrophages.(a) Expression of p65 in liver tissue was analyzed by IHC staining analysis. Representative views from each group were presented (original magnification, ×40). (b) The protein expression & phosphorylation of p65 at Ser 536 were observed in liver tissue & KCs isolated from the liver by WB. The results are shown as relative expression against control expression without treatment. Values represent means ± SD. (n = 4 in CD-fed group, n=6 in EtOH-fed group) *P < 0.05, **P < 0.01 vs liver tissues of CD-fed group. #P < 0.05, ##P < 0.01 vs KCs of CD-fed group. (c) p65 protein expression & phosphorylation were analyzed in total cell lysates of M0, M1 & M2 macrophages by WB. The results are shown as relative expression against control expression without treatment. Data shown are the mean ± SD from 3 independent experiments. *P < 0.05, **P < 0.01 vs control. (d) Representative colocalization of TERT with macrophage p65 immunoreactivity in liver tissue by using the double immunofluorescent (IF) analysis. (e) Effect of TERT on p65 expression & activation in LPS-stimulated RAW 264.7 cells. Expression of p65 & phosphorylated p65 were determined by WB. (f) Effect of PDTC on p65 expression in LPS-stimulated RAW 264.7 cells. Expression of p65 was determined by WB. (g) Expression of TERT upon treatment with PDTC was determined by WB in LPS-stimulated RAW 264.7 cells. (h) Effect of PDTC on the expression of M1 macrophage biomarkers in LPS-stimulated RAW 264.7 cells. The results are shown as relative expression against control expression without treatment. Data shown are the mean ± SD from 3 independent experiments. *P < 0.05, **P < 0.01 vs control. #P < 0.05, ##P < 0.01 vs LPS-treated group. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26725521), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: TERT Antibody (2C4) [NB100-317] -

Western Blot: TERT Antibody (2C4) [NB100-317] - Reduced expression levels of IFI16 protein in human normal diploid fibroblasts after treatment with histone deacetylase inhibitor are associated with increased expression of hTERT & increased telomerase activity.(A) Total RNA isolated from untreated (control, lane 1) or CGK1026 (10 µM for 24 h, lane 2) treated young WI-38 fibroblasts was subjected cDNA synthesis followed by semi-quantitative PCR using a pair of primer specific to the IFI16, hTERT, or actin. As a positive control, we used RNA from human HT1080, a human fibrosarcoma cell line. (B) Total RNA isolated from untreated (control) or CGK1026 (10 µM for 24 h; treated) treated young WI-38 fibroblasts was subjected cDNA synthesis, followed by quantitative real-time PCR using the TaqMan assay for the hTERT gene. Results are mean values of triplicate experiments & error bars represent standard deviation (**p<0.005). (C & D) Total protein extracts prepared from untreated (lane 1) or CGK1026 (10 µM for 24 h; treated) treated young WI-38 fibroblasts were subjected to immunoblotting using antibodies specific to the indicated proteins. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0008569), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.

Western Blot: TERT Antibody (2C4) [NB100-317] -

Western Blot: TERT Antibody (2C4) [NB100-317] - Plastic expression of TERT in murine macrophages.RAW264.7 cells were treated with LPS (1 μg/mL) for 24 h to polarize M1 macrophage phenotype, while treatment with IL-4 (15 ng/mL) for 24 h induced M2 macrophage phenotype. One population into another was transformed by culturing M1 macrophages with IL-4 & M2 macrophages with LPS, respectively. (a) The mRNA levels of M1 macrophage markers (TNF-alpha, IL-1 beta, CCL2 & NOS2) & M2 macrophage markers (Arg-1, IL-10, Mrc2 & CD163) were analyzed by real-time PCR. (b) The plastic expression of TERT in murine macrophage polarization was determined by real-time PCR & western blot. The results are shown as relative expression against control expression without treatment. Data shown are the mean ± SD from 3 independent experiments. *P < 0.05, **P < 0.01 vs control. (c) The expression of TERT in RAW264.7 macrophages polarization was analyzed by immunofluorescence (IF) assay. Representative views from each group were presented (original magnification, ×20). (d) RAW264.7 cells were treated with IFN-gamma (10 ng/mL) for 24 h alone or in combination with LPS. The production of TERT was determined by real-time PCR & western blot. The results are shown as relative expression against control expression without treatment. Data shown are the mean ± SD from 3 independent experiments. *P < 0.05, **P < 0.01 vs control. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26725521), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: TERT Antibody (2C4) [NB100-317] -

Western Blot: TERT Antibody (2C4) [NB100-317] - Reduced expression levels of IFI16 protein in human normal diploid fibroblasts after treatment with histone deacetylase inhibitor are associated with increased expression of hTERT & increased telomerase activity.(A) Total RNA isolated from untreated (control, lane 1) or CGK1026 (10 µM for 24 h, lane 2) treated young WI-38 fibroblasts was subjected cDNA synthesis followed by semi-quantitative PCR using a pair of primer specific to the IFI16, hTERT, or actin. As a positive control, we used RNA from human HT1080, a human fibrosarcoma cell line. (B) Total RNA isolated from untreated (control) or CGK1026 (10 µM for 24 h; treated) treated young WI-38 fibroblasts was subjected cDNA synthesis, followed by quantitative real-time PCR using the TaqMan assay for the hTERT gene. Results are mean values of triplicate experiments & error bars represent standard deviation (**p<0.005). (C & D) Total protein extracts prepared from untreated (lane 1) or CGK1026 (10 µM for 24 h; treated) treated young WI-38 fibroblasts were subjected to immunoblotting using antibodies specific to the indicated proteins. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0008569), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.

Western Blot: TERT Antibody (2C4) [NB100-317] -

Western Blot: TERT Antibody (2C4) [NB100-317] - Effect of alcohol on TERT expression in liver tissues & KCs during ALD development.(a) TERT expression in liver tissues was performed by IHC analysis. Representative views from each group were presented (original magnification, ×40). (b) Total TERT mRNA & protein levels in liver tissue were analyzed by real-time PCR & western blot. The results are shown as relative expression against control expression without treatment. (c) Representative colocalization of TERT with macrophage CD68 immunoreactivity in liver tissue by using the double immunofluorescent (IF) analysis. (d) Total TERT mRNA & protein levels in KCs isolated from the liver were analyzed by real-time PCR & western blot. The results are shown as relative expression against control expression without treatment. (e) Quantification of telomerase activity (TA) in CD-fed mice & EtOH-fed mice. RNase treatment or heat inactivation of KCs isolated from the liver of EtOH-fed mice served as negative controls for the TA assay. All quantitative data are presented as mean ± SD percentage increase compared with CD-fed group (n = 4 in CD-fed group, n = 6 in EtOH-fed group) *P < 0.05, **P < 0.01 vs CD-fed group. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26725521), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: TERT Antibody (2C4) [NB100-317] -

Western Blot: TERT Antibody (2C4) [NB100-317] - Effect of TERT silencing on murine M1 macrophage polarization.TERT siRNA & GV144-TERT were transiently transfected into LPS-treated RAW264.7 cells, respectively. (a) The endogeous TERT levels were detected by real-time PCR & western blot. The results are shown as relative expression against control expression without treatment. (b) The mRNA levels of M1 macrophages biomarkers including TNF-alpha, IL-1 beta, NOS2 & CCL2 were detected by real-time PCR. The results are shown as relative expression against control expression without treatment. (c) The secretion of proinflammatory cytokines including TNF-alpha, IL-1 beta, IL-6 & IL-12 were determined by ELISA. (d) TERT successful over-expression was verified by real-time PCR & western blot in LPS-stimulated RAW 264.7 cells. The results are shown as relative expression against control expression without treatment. (e) The mRNA levels of M1 macrophages biomarkers were detected by real-time PCR. The results are shown as relative expression against control expression without treatment. (f) The secretion of proinflammatory cytokines were determined by ELISA. Data shown are the mean ± SD from 3 independent experiments. *P < 0.05, **P < 0.01 vs control group. #P < 0.05, ##P < 0.01 vs LPS-treated group. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26725521), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: TERT Antibody (2C4) [NB100-317] -

Western Blot: TERT Antibody (2C4) [NB100-317] - IFI16 inhibits c-Myc-stimulated transcription & hTERT expression in HeLa cells.(A) Total protein extracts prepared from HeLa cells infected with control retrovirus (lane 1) or a virus encoding IFI16 protein (lane 2) were subjected to immunoblotting using antibodies specific to the indicated proteins. (B) Sub-confluent cultures of HeLa cells were transfected with pMyc-TA-luc reporter plasmid (1.0 µg) along with a second pRL-TK reporter plasmid (0.2 µg) & an empty plasmid (pCMV; column 1), a plasmid encoding c-Myc (column 2 & 4), a plasmid encoding IFI16 (column 3), or both plasmids encoding c-Myc & increasing amounts of the plasmid encoding IFI16 protein (column 5 & 6). After 44–48 h of transfections, cells were lysed & the lysates were analyzed for dual luciferase activity. Normalized relative luciferase activity in control cells is indicated as 1.0. (C) Total protein extracts prepared from HeLa cells infected with control retrovirus (lane 1) or a virus encoding antisense to IFI16 mRNA (lanes 2) were subjected to immunoblotting using antibodies specific to the indicated proteins. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0008569), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.

Western Blot: TERT Antibody (2C4) [NB100-317] -

Western Blot: TERT Antibody (2C4) [NB100-317] - Effect of alcohol on TERT expression in liver tissues & KCs during ALD development.(a) TERT expression in liver tissues was performed by IHC analysis. Representative views from each group were presented (original magnification, ×40). (b) Total TERT mRNA & protein levels in liver tissue were analyzed by real-time PCR & western blot. The results are shown as relative expression against control expression without treatment. (c) Representative colocalization of TERT with macrophage CD68 immunoreactivity in liver tissue by using the double immunofluorescent (IF) analysis. (d) Total TERT mRNA & protein levels in KCs isolated from the liver were analyzed by real-time PCR & western blot. The results are shown as relative expression against control expression without treatment. (e) Quantification of telomerase activity (TA) in CD-fed mice & EtOH-fed mice. RNase treatment or heat inactivation of KCs isolated from the liver of EtOH-fed mice served as negative controls for the TA assay. All quantitative data are presented as mean ± SD percentage increase compared with CD-fed group (n = 4 in CD-fed group, n = 6 in EtOH-fed group) *P < 0.05, **P < 0.01 vs CD-fed group. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26725521), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: TERT Antibody (2C4) [NB100-317] -

Western Blot: TERT Antibody (2C4) [NB100-317] - Effect of alcohol on TERT expression in vitro.Acute alcohol treatment of RAW 264.7 cells can be achieved with 25 mM EtOH for 24 h. (a) TERT mRNA & protein expression in EtOH-stimulated RAW 264.7 cells were analyzed by real-time PCR & western blot. The results are shown as relative expression against control expression without treatment. The values represent means ± SD. *P < 0.05, **P < 0.01 vs control. #P<0.05, ##P<0.01 vs EtOH-treated group. (b) Effect of alcohol on M1 macrophage markers (TNF-alpha, IL-1 beta, CCL2 & NOS2) in RAW 264.7 cells without or with LPS stimulation. (c) Effect of alcohol on M2 macrophage markers (Arg-1, IL-10, Mrc2 & CD163) in RAW 264.7 cells without or with LPS stimulation. (d) Effect of alcohol on the production of cytokines including TNF-alpha, IL-1 beta, IL-6, IL-12 & IL-10 in RAW 264.7 cells without or with LPS stimulation. The results are shown as line chart. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26725521), licensed under a CC-BY license. Not internally tested by Novus Biologicals.