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Tetraspanin-4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-59438

Novus Biologicals, part of Bio-Techne
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NBP1-59438

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Synthetic peptides corresponding to TSPAN4 (tetraspanin 4) The peptide sequence was selected from the middle region of TSPAN4) Peptide sequence YTDKIDRYAQQDLKKGLHLYGTQGNVGLTNAWSIIQTDFRCCGVSNYTDW (NP_001020405). The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for Tetraspanin-4 Antibody

Western Blot: Tetraspanin-4 Antibody [NBP1-59438]

Western Blot: Tetraspanin-4 Antibody [NBP1-59438]

Western Blot: Tetraspanin-4 Antibody [NBP1-59438] - Jurkat cell lysate, concentration 0.2-1 ug/ml.
Immunohistochemistry: Tetraspanin-4 Antibody [NBP1-59438]

Immunohistochemistry: Tetraspanin-4 Antibody [NBP1-59438]

Immunohistochemistry: Tetraspanin-4 Antibody [NBP1-59438] - Human Adult Liver Observed Staining: Cytoplasm in hepatocytes Primary Antibody Concentration: 1 : 600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1 : 200 Magnification: 20X Exposure Time: 0.5 2.0 sec.
Western Blot: Tetraspanin-4 Antibody [NBP1-59438]

Western Blot: Tetraspanin-4 Antibody [NBP1-59438]

Western Blot: Tetraspanin-4 Antibody [NBP1-59438] - GST-Teraspanin EC2 Expressed in E. coli.

Applications for Tetraspanin-4 Antibody

Application
Recommended Usage

Western Blot

1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TSPAN4

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein and is similar in sequence to its family member CD53 antigen. It is known to complex with integrins and other transmembrane 4 superfamily proteins. Alternatively spliced transcript variants encoding different isoforms have been identified.

Long Name

Tetraspanin 4

Alternate Names

NAG2, Tetraspanin-4, TM4SF7

Entrez Gene IDs

7106 (Human)

Gene Symbol

TSPAN4

UniProt

Additional TSPAN4 Products

Product Documents for Tetraspanin-4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Tetraspanin-4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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