TFF1/pS2 Antibody (TFF1/2133) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08247
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # TFF1/2133
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant full-length human TFF1/pS2 protein (Uniprot: P04155)
Localization
Cytoplasmic
Specificity
It recognizes a polypeptide of 6.5kDa, identified as pS2 estrogen-regulated protein. Its epitope is located in the c-terminus of human pS2 protein. pS2 is a trefoil peptide. Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. These peptides contain three intra-chain disulfide bonds, forming the trefoil motif, or P-domain. pS2 is known to form dimers and this dimerization is thought to play a role in its protective and healing properties. About 60% of breast carcinomas are positive for pS2. Staining is cytoplasmic, often with localization to the Golgi apparatus. pS2 is shown to be localized in normal stomach mucosa, gastric fluid, goblet cells in the colon and small intestine, and in ulcerations of the gastrointestinal tract. Several studies have shown that pS2 is primarily expressed in estrogen receptor-positive breast tumors and it may define a subset of estrogen-dependent tumors that displays an increased likelihood of response to endocrine therapy.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
6.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07341).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for TFF1/pS2 Antibody (TFF1/2133) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: TFF1/pS2 Antibody (TFF1/2133) - Azide and BSA Free [NBP3-08247]
Immunocytochemistry/Immunofluorescence: TFF1/pS2 Antibody (TFF1/2133) [NBP3-08247] - Immunofluorescence staining of paraformaldehyde-fixed MCF-7 cells with TFF1/pS2 Mouse Monoclonal Antibody (TFF1/2133) followed by goat anti- Mouse IgG-CF488 (Green). Nucleus is labeled with RedDot(Red).Immunohistochemistry-Paraffin: TFF1/pS2 Antibody (TFF1/2133) - Azide and BSA Free [NBP3-08247]
Immunohistochemistry-Paraffin: TFF1/pS2 Antibody (TFF1/2133) [NBP3-08247] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with TFF1/pS2 Mouse Monoclonal Antibody (TFF1/2133).Flow Cytometry: TFF1/pS2 Antibody (TFF1/2133) - Azide and BSA Free [NBP3-08247]
Flow Cytometry: TFF1/pS2 Antibody (TFF1/2133) [NBP3-08247] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using TFF1/pS2 Mouse Monoclonal Antibody (TFF1/2133) followed by goat anti- Mouse IgG-CF488 (Blue); Isotype Control (Red).Applications for TFF1/pS2 Antibody (TFF1/2133) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: TFF1
Long Name
Trefoil Factor 1
Alternate Names
BCEI, D21S21, HPS2, PNR-2, PS2
Gene Symbol
TFF1
Additional TFF1 Products
Product Documents for TFF1/pS2 Antibody (TFF1/2133) - Azide and BSA Free
Product Specific Notices for TFF1/pS2 Antibody (TFF1/2133) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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