Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all TFII-I Antibodies »

Product Specifications

Immunogen

A synthetic peptide, which represented a portion of human general transcription factor II-I encoded within exons 32 and 33 (LocusLink ID 2969).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TFII-I Antibody

Western Blot: TFII-I Antibody [NB500-157]

Western Blot: TFII-I Antibody [NB500-157]

Western Blot: TFII-I Antibody [NB500-157] - Detection of Human GTF2I/TFII-I by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GTF2I/TFII-I antibody NB500-157 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry: TFII-I Antibody [NB500-157]

Immunohistochemistry: TFII-I Antibody [NB500-157]

Immunohistochemistry: TFII-I Antibody [NB500-157] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-GFT2I/TF II-I used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Western Blot: TFII-I Antibody [NB500-157]

Western Blot: TFII-I Antibody [NB500-157]

Western Blot: TFII-I Antibody [NB500-157] - Whole cell lysate (100 ug) from HEK293 cells treated with GTF2I/TFII-I siRNA (+) or untreated (-). Antibody used at 0.2 ug/ml.

Applications for TFII-I Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:1000-1:10000
Application Notes
By Western Blot, this antibody recognizes a band at ~135 kDa, representing TFII-I. *The investigator should determine the optimal working dilution for a specific application.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TFII-I

TFII-I is an unusual transcription factor possessing both basal and signal-induced transcriptional functions. It is a founding member of a family of transcription factors characterized by the presence of multiple helix-loop-helix repeat domains. It is involved in histone modification and SUMOylation. TFII-I expression also plays a role in preimplantation development and has been detected in the cytoplasm and nuclei of post-fertilization through first cleavage stage embryos.

Alternate Names

BAP-135,135kD, BAP135WBS, Bruton tyrosine kinase-associated protein 135, BTKAP1, DIWS, FLJ38776, FLJ56355, general transcription factor II, i, general transcription factor IIi, general transcription factor II-I, GTFII-I, IB291, SPINBTK-associated protein 135, TFII-ISRF-Phox1-interacting protein, WBSCR6, Williams-Beuren syndrome chromosome region 6

Entrez Gene IDs

2969 (Human)

Gene Symbol

GTF2I

Additional TFII-I Products

Product Documents for TFII-I Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TFII-I Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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