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TFII-I Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90010

Novus Biologicals, part of Bio-Techne
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NBP1-90010-25ul
NBP1-90010

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (100%), Rat (99%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FVIKKHELLNSTREDLQLDKPASGVKEEWYARITKLRKMVDQLFCKKFAEALGSTEAKAVPYQKFEAHPNDLYVEGLPENIPFRSPSWYGIPRLEKIIQVGNRIKFVIKRPELLTHSTTEVTQPRTNTPVKEDWNVRIT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TFII-I Antibody

Western Blot: TFII-I Antibody [NBP1-90010]

Western Blot: TFII-I Antibody [NBP1-90010]

Western Blot: TFII-I Antibody [NBP1-90010] - Analysis in human cell line SH-SY5Y.
Immunocytochemistry/ Immunofluorescence: TFII-I Antibody [NBP1-90010]

Immunocytochemistry/ Immunofluorescence: TFII-I Antibody [NBP1-90010]

Immunocytochemistry/Immunofluorescence: TFII-I Antibody [NBP1-90010] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TFII-I Antibody [NBP1-90010]

Immunohistochemistry-Paraffin: TFII-I Antibody [NBP1-90010]

Immunohistochemistry-Paraffin: TFII-I Antibody [NBP1-90010] - Staining of human stomach shows strong nuclear positivity in glandular cells.

Applications for TFII-I Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 5 using NBP1-90010 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TFII-I

TFII-I encodes a multifunctional phosphoprotein with roles in transcription and signal transduction. It is deleted in Williams-Beuren syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at chromosome 7q11.23. The exon(s) encoding 5' UTR has not been fully defined, but this gene is known to contain at least 34 exons, and its alternative splicing generates 4 transcript variants.

Alternate Names

BAP-135,135kD, BAP135WBS, Bruton tyrosine kinase-associated protein 135, BTKAP1, DIWS, FLJ38776, FLJ56355, general transcription factor II, i, general transcription factor IIi, general transcription factor II-I, GTFII-I, IB291, SPINBTK-associated protein 135, TFII-ISRF-Phox1-interacting protein, WBSCR6, Williams-Beuren syndrome chromosome region 6

Gene Symbol

GTF2I

Additional TFII-I Products

Product Documents for TFII-I Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TFII-I Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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