Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all TFIIB Antibodies »

Product Summary for TFIIB Antibody

Immunogen

The immunogen for this product maps to a region between residue 256 and 316 of human General Transcription Factor IIB using the numbering given in entry NP_001505.1 (GeneID 2959).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TFIIB Antibody

Immunoprecipitation: TFIIB Antibody [NBP1-49982]

Immunoprecipitation: TFIIB Antibody [NBP1-49982]

Immunoprecipitation: TFIIB Antibody [NBP1-49982] - Detection of Human GTF2B/ TFIIB by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti- GTF2B/ TFIIB antibody used for IP at 3 mcg/mg lysate. GTF2B/ TFIIB was also immunoprecipitated by rabbit anti-TFIIB antibody which recognizes an upstream epitope. For blotting immunoprecipitated GTF2B/ TFIIB was used at 1 mcg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Applications for TFIIB Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TFIIB

This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II.

Alternate Names

general transcription factor IIB, General transcription factor TFIIB, S300-II, TFIIBTF2BRNA polymerase II transcription factor IIB, transcription initiation factor IIB

Gene Symbol

GTF2B

Additional TFIIB Products

Product Documents for TFIIB Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TFIIB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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