TFIIB Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34143

Novus Biologicals, part of Bio-Techne
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NBP2-34143
NBP2-34143-25ul
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TFIIB Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: LPRDTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFG

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (85%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TFIIB Antibody

Immunohistochemistry-Paraffin: TFIIB Antibody [NBP2-34143]

Immunohistochemistry-Paraffin: TFIIB Antibody [NBP2-34143]

Immunohistochemistry-Paraffin: TFIIB Antibody [NBP2-34143] - Staining in human gallbladder and pancreas tissues using anti-GTF2B antibody. Corresponding GTF2B RNA-seq data are presented for the same tissues.
Western Blot: TFIIB Antibody [NBP2-34143]

Western Blot: TFIIB Antibody [NBP2-34143]

Western Blot: TFIIB Antibody [NBP2-34143] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-GTF2B antibody. Remaining relative intensity is presented.
Immunocytochemistry/ Immunofluorescence: TFIIB Antibody [NBP2-34143]

Immunocytochemistry/ Immunofluorescence: TFIIB Antibody [NBP2-34143]

Immunocytochemistry/Immunofluorescence: TFIIB Antibody [NBP2-34143] - Staining of human cell line HEK 293 shows localization to nucleus & nuclear bodies. Antibody staining is shown in green.
View 2 more images

Applications for TFIIB Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TFIIB

TFIIB encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. [provided by RefSeq]

Alternate Names

general transcription factor IIB, General transcription factor TFIIB, S300-II, TFIIBTF2BRNA polymerase II transcription factor IIB, transcription initiation factor IIB

Gene Symbol

GTF2B

UniProt

Q00403 (Human)

Additional TFIIB Products

Product Documents for TFIIB Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TFIIB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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