TFIIE-alpha Antibody

Novus Biologicals, part of Bio-Techne | Catalog # H00002960-B01P

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Mouse IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TFIIE-alpha Antibodies »

Product Specifications

Immunogen

GTF2E1 (NP_005504.2, 1 a.a. - 439 a.a.) full-length human protein. MADPDVLTEVPAALKRLAKYVIRGFYGIEHALALDILIRNSCVKEEDMLELLKFDRKQLRSVLNNLKGDKFIKCRMRVETAADGKTTRHNYYFINYRTLVNVVKYKLDHMRRRIETDERDSTNRASFKCPVCSSTFTDLEANQLFDPMTGTFRCTFCHTEVEEDESAMPKKDARTLLARFNEQIEPIYALLRETEDVNLAYEILEPEPTEIPALKQSKDHAATTAGAASLAGGHHREAWATKGPSYEDLYTQNVVINMDDQEDLHRASLEGKSAKERPIWLRESTVQGAYGSEDMKEGGIDMDAFQEREEGHAGPDDNEEVMRALLIHEKKTSSAMAGSVGAAAPVTAANGSDSESETSESDDDSPPRPAAVAVHKREEDEEEDDEFEEVADDPIVMVAGRPFSYSEVSQRPELVAQMTPEEKEAYIAMGQRMFEDLFE

Clonality

Polyclonal

Host

Mouse

Isotype

IgG

Description

Quality control test: Antibody reactive against mammalian transfected lysate.

Scientific Data Images for TFIIE-alpha Antibody

Western Blot: TFIIE-alpha Antibody [H00002960-B01P]

Western Blot: TFIIE-alpha Antibody [H00002960-B01P]

Western Blot: TFIIE-alpha Antibody [H00002960-B01P] - Analysis of GTF2E1 expression in transfected 293T cell line by GTF2E1 polyclonal antibody. Lane 1: GTF2E1 transfected lysate(48.29 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: TFIIE-alpha Antibody [H00002960-B01P]

Immunocytochemistry/ Immunofluorescence: TFIIE-alpha Antibody [H00002960-B01P]

Immunocytochemistry/Immunofluorescence: TFIIE-alpha Antibody [H00002960-B01P] - Analysis of purified antibody to GTF2E1 on HepG2 cell. (antibody concentration 10 ug/ml)

Applications for TFIIE-alpha Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
It has been used for WB, Functional and IF.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4)

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TFIIE-alpha

Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase andDNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase

Alternate Names

FE, General transcription factor IIE 56 kDa subunit, general transcription factor IIE subunit 1, general transcription factor IIE, polypeptide 1 (alpha subunit, 56kD), general transcription factor IIE, polypeptide 1, alpha 56kDa, TF2E1, TFIIE-A, TFIIE-alpha, Transcription initiation factor IIE subunit alpha

Gene Symbol

GTF2E1

UniProt

NP_005504.2 (Human)

Additional TFIIE-alpha Products

Product Documents for TFIIE-alpha Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TFIIE-alpha Antibody

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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