TFIIE-alpha Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46196
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (100%), Primate (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 389 and 439 of human General Transcription Factor IIE, polypeptide 1, alpha, using the numbering given in entry NP_005504.2 (GeneID 2960).
Reactivity Notes
Orangutan (100%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for TFIIE-alpha Antibody
![Western Blot: TFIIE-alpha Antibody [NBP1-46196]](https://resources.bio-techne.com/images/products/TFIIE-alpha-Antibody-Western-Blot-NBP1-46196-img0001.jpg "Western Blot: TFIIE-alpha Antibody [NBP1-46196]")
Western Blot: TFIIE-alpha Antibody [NBP1-46196]
Western Blot: TFIIE-alpha Antibody [NBP1-46196] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-TFIIE-alpha antibody NBP1-46196 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. TFIIE-alpha was also immunoprecipitated by rabbit anti-TFIIE-alpha antibody NBP1-46195, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).![Immunohistochemistry-Paraffin: TFIIE-alpha Antibody [NBP1-46196]](https://resources.bio-techne.com/images/products/TFIIE-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-46196-img0002.jpg "Immunohistochemistry-Paraffin: TFIIE-alpha Antibody [NBP1-46196]")
Immunohistochemistry-Paraffin: TFIIE-alpha Antibody [NBP1-46196]
Immunohistochemistry-Paraffin: TFIIE-alpha Antibody [NBP1-46196] - Section of human colon carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- GTF2E1/TFIIE-alpha used at a dilution of 1:200 (1ug/ml). Detection: DABApplications for TFIIE-alpha Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:100 - 1:500
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: TFIIE-alpha
Alternate Names
FE, General transcription factor IIE 56 kDa subunit, general transcription factor IIE subunit 1, general transcription factor IIE, polypeptide 1 (alpha subunit, 56kD), general transcription factor IIE, polypeptide 1, alpha 56kDa, TF2E1, TFIIE-A, TFIIE-alpha, Transcription initiation factor IIE subunit alpha
Gene Symbol
GTF2E1
Additional TFIIE-alpha Products
Product Documents for TFIIE-alpha Antibody
Product Specific Notices for TFIIE-alpha Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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