TFIP11 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-40364

Novus Biologicals, part of Bio-Techne
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NBP1-40364
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Chimpanzee (100%), Monkey (100%), Mouse (100%), Orangutan (100%), Rabbit (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all TFIP11 Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 1 and 50 of human tuftelin interacting protein 11 using the numbering given in entry NP_036275.1 (GeneID 24144).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TFIP11 Antibody

Western Blot: TFIP11 Antibody [NBP1-40364]

Western Blot: TFIP11 Antibody [NBP1-40364]

Western Blot: TFIP11 Antibody [NBP1-40364] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TFIP11 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. TFIP11 was also immunoprecipitated by rabbit anti-TFIP11 antibody NBP1-40365 which recognizes a downstream epitope.
Immunohistochemistry: TFIP11 Antibody [NBP1-40364]

Immunohistochemistry: TFIP11 Antibody [NBP1-40364]

Immunohistochemistry: TFIP11 Antibody [NBP1-40364] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- TFIP11 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Applications for TFIP11 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TFIP11

Neurotensin (NT) initiates an intracellular response by interacting with the G protein-coupled receptors NTR1 (NTS1 receptor, high affinity NTR) and NTR2 (NTS2 receptor, levocabastine-sensitive neurotensin receptor), and the type I receptor NTR3 (NTS3 receptor, sortilin-1, Gp95). NT has a wide distribution in regions of the brain and in peripheral tissues where NT receptors can contribute to hypotension, hyperglycemia, hypothermia, antinociception and regulation of intestinal motility and secretion. HL-60 cells express NTR1, which can couple to Gq, Gi/o, or Gs. Alternative splicing of rat NTR2 can generate a 5-transmembrane domain variant isoform that is co-expressed with the full-length NTR2 throughout the brain and spinal cord. NTR3 activation in the murine microglial cell line N11 induces MIP-2, MCP-1, IL-1beta and TNFalpha in an ERK1/2 and Akt kinase-dependent manner.

Alternate Names

bK445C9.6, DKFZP434B194, FLJ22086, hNtr1, NTR1, Septin and tuftelin-interacting protein 1, STIP, STIP-1, TIP39Spp382, tuftelin interacting protein 11, tuftelin-interacting protein 11

Entrez Gene IDs

24144 (Human)

Gene Symbol

TFIP11

Additional TFIP11 Products

Product Documents for TFIP11 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TFIP11 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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