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TfR (Transferrin R) Antibody (040)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89744

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 040

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human TfR (Transferrin R) (Accession#: AAA61153.1; Cys89-Phe760).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for TfR (Transferrin R) Antibody (040)

Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody (040) [NBP2-89744]

Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody (040) [NBP2-89744]

Immunocytochemistry/Immunofluorescence: TfR (Transferrin R) Antibody (040) [NBP2-89744] - Staining of Human TFRC in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human TFRC monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.
Flow Cytometry: TfR (Transferrin R) Antibody (040) [NBP2-89744]

Flow Cytometry: TfR (Transferrin R) Antibody (040) [NBP2-89744]

Flow Cytometry: TfR (Transferrin R) Antibody (040) [NBP2-89744] - Analysis of Human TFRC(CD71) expression on U937 cells. Cells were stained with purified anti-Human TFRC(CD71), then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Applications for TfR (Transferrin R) Antibody (040)

Application
Recommended Usage

Flow Cytometry

1:25-1:100

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TfR (Transferrin R)

Useful in studies of dividing haematopoietic and tumour cell populations, and metabolic activity.

Long Name

Transferrin Receptor

Alternate Names

CD71, TfR (TransferrinR), TFR1, TFRC, TRFR

Gene Symbol

TFRC

Additional TfR (Transferrin R) Products

Product Documents for TfR (Transferrin R) Antibody (040)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TfR (Transferrin R) Antibody (040)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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