TfR (Transferrin R) Antibody (66IG10)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32946
![Flow Cytometry: TfR (Transferrin R) Antibody (66IG10) [NBP2-32946]](https://resources.bio-techne.com/images/products/TfR-Transferrin-R-Antibody-66IG10-Flow-Cytometry-NBP2-32946-img0002.jpg "Flow Cytometry: TfR (Transferrin R) Antibody (66IG10) [NBP2-32946]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 66IG10
Concentration
0.2 mg/ml
Product Summary for TfR (Transferrin R) Antibody (66IG10)
Immunogen
Human thymocytes (T-cells)
Localization
Cell surface
Specificity
It recognizes a ~90-95kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa. This monoclonal antibody is highly specific to CD71 and shows no cross-reaction with other related proteins. Its epitope is localized in the extracellular domain of CD71. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34542)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for TfR (Transferrin R) Antibody (66IG10)
Flow Cytometry: TfR (Transferrin R) Antibody (66IG10) [NBP2-32946]
Flow Cytometry: TfR (Transferrin R) Antibody (66IG10) [NBP2-32946] - Flow Cytometric Analysis of Jurkat cells. TfR (Transferrin R) Antibody (66IG10) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).![Flow Cytometry: TfR (Transferrin R) Antibody (66IG10) [NBP2-32946]](https://resources.bio-techne.com/images/products/TfR-Transferrin-R-Antibody-66IG10-Flow-Cytometry-NBP2-32946-img0001.jpg "Flow Cytometry: TfR (Transferrin R) Antibody (66IG10) [NBP2-32946]")
Flow Cytometry: TfR (Transferrin R) Antibody (66IG10) [NBP2-32946]
Flow Cytometry: TfR (Transferrin R) Antibody (66IG10) [NBP2-32946] - Flow Cytometry of human CD71 on K562 cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled CD71 Monoclonal Antibody (66IG10).Applications for TfR (Transferrin R) Antibody (66IG10)
Application
Recommended Usage
ELISA
2-4 ug/ml
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: TfR (Transferrin R)
Long Name
Transferrin Receptor
Alternate Names
CD71, TfR (TransferrinR), TFR1, TFRC, TRFR
Gene Symbol
TFRC
UniProt
Additional TfR (Transferrin R) Products
Product Documents for TfR (Transferrin R) Antibody (66IG10)
Product Specific Notices for TfR (Transferrin R) Antibody (66IG10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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