TfR (Transferrin R) Antibody (DF1513)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32945
Clone DF1513 was used by HLDA to establish CD designation.
![Simple Western: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945]](https://resources.bio-techne.com/images/products/TfR-Transferrin-R-Antibody-DF1513-Simple-Western-NBP2-32945-img0001.jpg "Simple Western: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945]")
![Simple Western: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945]](https://resources.bio-techne.com/images/products/TfR-Transferrin-R-Antibody-DF1513-Simple-Western-NBP2-32945-img0002.jpg "Simple Western: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945]")
![Western Blot: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945]](https://resources.bio-techne.com/images/products/TfR-Transferrin-R-Antibody-DF1513-Western-Blot-NBP2-32945-img0006.jpg "Western Blot: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # DF1513
Concentration
0.2 mg/ml
Product Specifications
Immunogen
KG1 acute myeloid leukemia cell line
Localization
Cell surface
Specificity
It recognizes a ~90-95kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa (Workshop IV). This monoclonal antibody is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34602)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for TfR (Transferrin R) Antibody (DF1513)
Western Blot: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945]
Western Blot: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Western Blot Analysis of Jurkat, Raji, and THP-1 cell lysate using TfR (Transferrin R) Antibody (DF1513).![Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945]](https://resources.bio-techne.com/images/products/TfR-Transferrin-R-Antibody-DF1513-Immunocytochemistry-Immunofluorescence-NBP2-32945-img0005.jpg "Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945]")
Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945]
Immunocytochemistry/Immunofluorescence: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Immunofluorescence Analysis of Human Jurkat cells labeling CD71 with TfR (Transferrin R) Antibody (DF1513) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)![Flow Cytometry: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945]](https://resources.bio-techne.com/images/products/TfR-Transferrin-R-Antibody-DF1513-Flow-Cytometry-NBP2-32945-img0007.jpg "Flow Cytometry: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945]")
Flow Cytometry: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945]
Flow Cytometry: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Flow Cytometric Analysis of human Jurkat cells using TfR (Transferrin R) antibody (DF1513) followed by Goat anti-Mouse IgG-CF488 (Orange); cells alone (Blue); Isotype Control (Red).Applications for TfR (Transferrin R) Antibody (DF1513)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Simple Western
10 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using NBP2-32945 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: TfR (Transferrin R)
Long Name
Transferrin Receptor
Alternate Names
CD71, TfR (TransferrinR), TFR1, TFRC, TRFR
Gene Symbol
TFRC
UniProt
Additional TfR (Transferrin R) Products
Product Documents for TfR (Transferrin R) Antibody (DF1513)
Product Specific Notices for TfR (Transferrin R) Antibody (DF1513)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...