TfR (Transferrin R) Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-92243
![Western Blot: TfR (Transferrin R) AntibodyBSA Free [NB100-92243]](https://resources.bio-techne.com/images/products/Transferrin-Receptor-Antibody-Western-Blot-NB100-92243-img0001.jpg "Western Blot: TfR (Transferrin R) AntibodyBSA Free [NB100-92243]")
![Western Blot: TfR (Transferrin R) AntibodyBSA Free [NB100-92243]](https://resources.bio-techne.com/images/products/TfR-Transferrin-R-Antibody-Western-Blot-NB100-92243-img0006.jpg "Western Blot: TfR (Transferrin R) AntibodyBSA Free [NB100-92243]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Proximity Ligation Assay, Western Blot
Cited:
Block/Neutralize, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Proximity Ligation Assay, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Synthetic peptide made to an internal portion of human Transferrin R (between amino acids 20-75) [UniProt P02786]
Marker
Recycling Endosome Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
89 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for TfR (Transferrin R) Antibody - BSA Free
Western Blot: TfR (Transferrin R) AntibodyBSA Free [NB100-92243]
Western Blot: TfR (Transferrin R) Antibody [NB100-92243] - Analysis of TfR1 pAb at 1:500 dilution. (1) HEK293T whole cell lysate, (2) Raw264.7 whole cell lysate, and (3) H9C2 whole cell lysate.![Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody - BSA Free [NB100-92243]](https://resources.bio-techne.com/images/products/Transferrin-Receptor-Antibody-Immunocytochemistry-Immunofluorescence-NB100-92243-img0004.jpg "Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody - BSA Free [NB100-92243]")
Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody - BSA Free [NB100-92243]
Immunocytochemistry/Immunofluorescence: TfR (Transferrin R) Antibody [NB100-92243] - Transferrin Receptor Antibody [NB100-92243] - Transferrin receptor antibody was tested at 1:50 in HeLa cells with DylLght 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.![Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody - BSA Free [NB100-92243]](https://resources.bio-techne.com/images/products/TfR-Transferrin-R-Antibody-BSA-Free-Immunohistochemistry-Paraffin-NB100-92243-img0007.jpg "Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody - BSA Free [NB100-92243]")
Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody - BSA Free [NB100-92243]
Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody - BSA Free [NB100-92243] - Analysis of a FFPE tissue section of human placenta using 1:200 dilution of TfR (Transferrin R) antibody NB100-92243. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.![Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody - BSA Free [NB100-92243]](https://resources.bio-techne.com/images/products/TfR-Transferrin-R-Antibody-Immunohistochemistry-Paraffin-NB100-92243-img0005.jpg "Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody - BSA Free [NB100-92243]")
Applications for TfR (Transferrin R) Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
reported in scientific literature
Immunohistochemistry-Paraffin
1:50-1:200
Proximity Ligation Assay
reported in scientific literature (PMID 31091436)
Western Blot
1:500-1:1000
Application Notes
Western blot (WB) analysis of Transferrin Receptor pAb in extracts from 293 cells. Immunohistochemistry analyzes of Transferrin Receptor pAb in paraffin-embedded human brain tissue.
Reviewed Applications
Read 1 review rated 3 using NB100-92243 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TfR (Transferrin R)
Long Name
Transferrin Receptor
Alternate Names
CD71, TfR (TransferrinR), TFR1, TFRC, TRFR
Entrez Gene IDs
7037 (Human)
Gene Symbol
TFRC
UniProt
Additional TfR (Transferrin R) Products
Product Documents for TfR (Transferrin R) Antibody - BSA Free
Product Specific Notices for TfR (Transferrin R) Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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