TGF-alpha Antibody (MF9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34296
![Immunohistochemistry-Paraffin: TGF-alpha Antibody (MF9) [NBP2-34296]](https://resources.bio-techne.com/images/products/TGF-alpha-Antibody-MF9-Immunohistochemistry-Paraffin-NBP2-34296-img0001.jpg "Immunohistochemistry-Paraffin: TGF-alpha Antibody (MF9) [NBP2-34296]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Bovine
Cited:
Human
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # MF9
Concentration
0.2 mg/ml
Product Specifications
Immunogen
A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGF-alpha (exact sequence is proprietary) (Uniprot: P01135)
Localization
Cytoplasmic and Extracellular (secreted)
Specificity
This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with Ab-1 is completely blocked by the peptide used for raising this antibody. TGF (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34683)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for TGF-alpha Antibody (MF9)
Immunohistochemistry-Paraffin: TGF-alpha Antibody (MF9) [NBP2-34296]
Immunohistochemistry-Paraffin: TGF-alpha Antibody (MF9) [NBP2-34296] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma Stained with TGF alpha Monoclonal Antibody (MF9).Applications for TGF-alpha Antibody (MF9)
Application
Recommended Usage
ELISA
2-4 ug/ml
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
2-4 ug/ml
Western Blot
0.5-1.0ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 2-4ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 2-4ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: TGF-alpha
Long Name
Transforming Growth Factor alpha
Alternate Names
TGFA, TGFalpha
Gene Symbol
TGFA
UniProt
Additional TGF-alpha Products
Product Documents for TGF-alpha Antibody (MF9)
Product Specific Notices for TGF-alpha Antibody (MF9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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