TGF-alpha Antibody (tAb2) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81082
Recombinant Monoclonal Antibody
![Flow Cytometry: TGF-alpha Antibody (tAb2) - Chimeric - Azide and BSA Free [NBP2-81082]](https://resources.bio-techne.com/images/products/TGF-alpha-Antibody-tAb2-Flow-Cytometry-NBP2-81082-img0001.jpg "Flow Cytometry: TGF-alpha Antibody (tAb2) - Chimeric - Azide and BSA Free [NBP2-81082]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Dot Blot, ELISA, Flow Cytometry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # tAb2
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Biologically active hemocyanin-coupled human TGF-alpha.
Specificity
tAb2 binds the N-terminal sequence of TGF-alpha, VVSHFND (with residues 4 to 7 being the minimal binding epitope).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for TGF-alpha Antibody (tAb2) - Chimeric - Azide and BSA Free
Flow Cytometry: TGF-alpha Antibody (tAb2) - Chimeric - Azide and BSA Free [NBP2-81082]
Flow Cytometry: TGF-alpha Antibody (tAb2) [NBP2-81082] - Flow-cytometry using the [NBP2-81082]. Human peripheral blood leukocytes were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with unimmunized rabbit IgG antibody (isotype control, black line) or the rabbit IgG-chimeric version of tAb2 (NBP2-81082, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.Applications for TGF-alpha Antibody (tAb2) - Chimeric - Azide and BSA Free
Application
Recommended Usage
Dot Blot
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. Human transforming growth factora (TGFa) consists of 50 amino acid residues with three disulphide bridges. It belongs to the family of epidermal growth factor related peptides and binds to EGFreceptor. It is involved in cell proliferation in healthy cells and is found in various cancer cells. Transfection of Fibroblasts with the TGFa gene induces tumor formation.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: TGF-alpha
Long Name
Transforming Growth Factor alpha
Alternate Names
TGFA, TGFalpha
Gene Symbol
TGFA
Additional TGF-alpha Products
Product Documents for TGF-alpha Antibody (tAb2) - Chimeric - Azide and BSA Free
Product Specific Notices for TGF-alpha Antibody (tAb2) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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