TGF-beta 1 Antibody (OTI1E12) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-74495

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1E12

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all TGF-beta 1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human TGFB1(NP_000651) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

28.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TGF-beta 1 Antibody (OTI1E12) - Azide and BSA Free

Western Blot: TGF-beta 1 Antibody (OTI1E12)Azide and BSA Free [NBP2-74495]

Western Blot: TGF-beta 1 Antibody (OTI1E12)Azide and BSA Free [NBP2-74495]

Western Blot: TGF-beta 1 Antibody (OTI1E12) - Azide and BSA Free [NBP2-74495] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TGR-beta 1.
Immunocytochemistry/ Immunofluorescence: TGF-beta 1 Antibody (OTI1E12) - Azide and BSA Free [NBP2-74495]

Immunocytochemistry/ Immunofluorescence: TGF-beta 1 Antibody (OTI1E12) - Azide and BSA Free [NBP2-74495]

Immunocytochemistry/Immunofluorescence: TGF-beta 1 Antibody (OTI1E12) - Azide and BSA Free [NBP2-74495] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY TGF-Beta 1.
Flow Cytometry: TGF-beta 1 Antibody (OTI1E12) - Azide and BSA Free [NBP2-74495]

Flow Cytometry: TGF-beta 1 Antibody (OTI1E12) - Azide and BSA Free [NBP2-74495]

Flow Cytometry: TGF-beta 1 Antibody (OTI1E12) - Azide and BSA Free [NBP2-74495] - Analysis of SF295 cells, using TGFB1 antibody,(Red), compared to a nonspecific negative control antibody (Blue).
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Applications for TGF-beta 1 Antibody (OTI1E12) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:50

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TGF-beta 1

TGF-beta-1 is a multifunctional cytokine that belongs to a superfamily of structurally related regulatory proteins, which includes three mammalian TGF-beta isoforms (TGF-beta-1, -beta-2, and -beta-3), activin/inhibins and bone morphogenetic proteins. The most abundant isoform, TGF-beta-1, is a 25kDa homodimer composed of two 12.5kDa subunits joined by disulfide bonds. TGF-beta-1 is a highly conserved molecule - the amino acid sequence between human and mouse differ only by one residue. Although originally defined by its ability to cause anchorage independent cell growth and changes in cell morphology of rat fibroblasts, subsequent research has revealed that TGF-beta is actually a major growth inhibitor for most cell types. It is produced by a wide variety of cell and tissue types during all stages of cell differentiation. TGF-beta-1 sources include platelets, bone and soft tissues such as placenta and kidneys.

Long Name

Transforming Growth Factor beta 1

Alternate Names

TGF beta1, TGFB, TGFB1, TGFbeta 1

Gene Symbol

TGFB1

Additional TGF-beta 1 Products

Product Documents for TGF-beta 1 Antibody (OTI1E12) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TGF-beta 1 Antibody (OTI1E12) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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