TGF-beta 1, 2, 3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1835R
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Validated:
Multi-Species
Cited:
Human, Mouse
Applications
Validated:
Neutralization
Cited:
Cell Culture, Functional Assay, Neutralization
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # 1D11R
Product Specifications
Immunogen
Bovine bone-derived TGF-beta 1 and TGF-beta 2
Specificity
Detects bovine, chicken, mouse, and human TGF-beta in ELISAs and Western blots. It recognizes human TGF-beta 1, TGF-beta 2, and TGF-beta 3.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for TGF-beta 1, 2, 3 Antibody
TGF-beta 1 Inhibition of IL-4-dependent Cell Proliferation and Neutralization by TGF-beta 1, 2, 3 Antibody.
Recombinant Human TGF-beta 1 (Catalog # 240-B) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Recombinant Human TGF-beta 1 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-TGF-beta 1, 2, 3 Monoclonal Antibody (Catalog # MAB1835R). The ND50 is typically0.25-1.25 µg/mL.Catalog # 240-BApplications for TGF-beta 1, 2, 3 Antibody
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize TGF‑ beta1 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line. Tsang, M. et al. (1990) Lymphokine Res. 9:607. The Neutralization Dose (ND50) is typically 0.25-1.25 µg/mL in the presence of 1 ng/mL Recombinant Human TGF‑ beta1 and 7.5 ng/mL Recombinant Mouse IL‑4.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TGF-beta 1, 2, 3
References
- Ayala A. et al. (1992) FASEB J. 6:A1604.
- Roberts A.B. and Sporn M.B., eds. (1990) Peptide Growth Factors and Their Receptors I, Springer-Verlag, 419.
- Dasch J.R. et al. (1989) J. Immunol. 142:1536.
Long Name
Transforming Growth Factor beta 1, 2, and 3
Additional TGF-beta 1, 2, 3 Products
Product Documents for TGF-beta 1, 2, 3 Antibody
Product Specific Notices for TGF-beta 1, 2, 3 Antibody
For research use only
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