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TGF-beta 1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2402

R&D Systems, part of Bio-Techne
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MAB2402-100
MAB2402-SP

Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Feline

Applications

Validated:

ELISA Capture (Matched Antibody Pair)

Cited:

Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 9016EC

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant TGF-beta 1 and latent TGF-beta 1

Specificity

Detects TGF-beta 1 from human, mouse, rat, and other species in direct ELISAs and Western blots. In sandwich ELISAs, less than2% cross-reactivity with recombinant human (rh) TGF‑ beta3 and recombinant amphibian TGF‑ beta5 and no cross-reactivity with recombinant porcine TGF-beta 2 or rhTGF-beta 2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for TGF-beta 1 Antibody

Application
Recommended Usage

Human TGF-beta 1 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: TGF-beta 1 Biotinylated Antibody (Catalog # BAF240)
  • Standard: Recombinant Human TGF-beta 1 Protein (Catalog # 240-B)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta 1

TGF-beta 1, -2, and -3 are a closely related group of proteins (70-80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta 1, -2, and -3 are disulfide-linked homodimers.

Long Name

Transforming Growth Factor beta 1

Alternate Names

TGF beta1, TGFB, TGFB1, TGFbeta 1

Entrez Gene IDs

7040 (Human); 21803 (Mouse); 59086 (Rat); 397078 (Porcine); 100033900 (Equine)

Gene Symbol

TGFB1

Additional TGF-beta 1 Products

Product Documents for TGF-beta 1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for TGF-beta 1 Antibody

For research use only

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