TGF-beta 3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB643
Key Product Details
Species Reactivity
Validated:
Multi-Species
Cited:
Human
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Immunohistochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 44922
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TGF-beta 3
Ala301-Ser412 (Tyr340Phe)
Accession # NP_003230.1
Ala301-Ser412 (Tyr340Phe)
Accession # NP_003230.1
Specificity
Detects TGF-beta 3 from multiple species in ELISAs and Western blots. In ELISAs, does not cross-react with recombinant human (rh) TGF-beta 1or rhTGF-beta 2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for TGF-beta 3 Antibody
TGF-beta 3 in Human Heart.
TGF-beta 3 was detected in immersion fixed paraffin-embedded sections of human heart using TGF-beta 3 Monoclonal Antibody (Catalog # MAB643) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for TGF-beta 3 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human heart
Sample: Immersion fixed paraffin-embedded sections of human heart
Western Blot
1 µg/mL
Sample: Recombinant Human TGF-beta 3 (Catalog # 243-B3)
under non-reducing conditions only
Sample: Recombinant Human TGF-beta 3 (Catalog # 243-B3)
under non-reducing conditions only
Human TGF-beta 3 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TGF-beta 3
Long Name
Transforming Growth Factor beta 3
Alternate Names
ARVD1, LDS5, RNHF, TGFB3, TGFbeta 3
Gene Symbol
TGFB3
UniProt
Additional TGF-beta 3 Products
Product Documents for TGF-beta 3 Antibody
Product Specific Notices for TGF-beta 3 Antibody
For research use only
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