TGN38 Antibody (2F7.1)

Immunocytochemistry/ Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575]

Immunocytochemistry/Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575] - HeLa and COS7 cells were rinsed with PBS and fixed with -20C methanol for 5 minutes. Cells were incubated with anti-TGN38 antibody diluted 1:100 in PBS containing 0.2% BSA for 1 hour. The cells were then rinsed in PBS and incubated with goat anti-mouse IgG conjugated to rhodamine for 30 minutes in PBS containing 0.2% BSA. Following washing, cells were imaged using a Zeiss Axioplan microscope. ICC/IF image submitted by a verified customer review.

Immunocytochemistry/ Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575]

Immunocytochemistry/Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575] - Staining of TGN-38 in NRK cells.

Immunocytochemistry/ Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575]

Immunocytochemistry/Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575] - Analysis of Phalloidin (green) and TGN38 (purple) in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a TGN38 monoclonal antibody at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 680 goat anti-mouse IgG secondary antibody at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 488 Phalloidin at a dilution of 1:300 (1 unit/mL final concentration) for 30 minutes. Images were taken on a Thermo Scientific ArrayScan VTI at 20X magnification.