TGN46 Antibody (9G5X6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15817

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15817-100ul
NBP3-15817-20ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9G5X6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TGN46 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TGN46 (O43493). MRFVVALVLLNVAAAGAVPLLATESVKQEEAGVRPSAGNVSTHPSLSQRPGGSTKSHPEPQTPKDSPSKSSAEAQTPEDTPNKSGAEAKTQKDSSNKSGA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Observed MW 90-100kDa Calculated MW-46kDa

Scientific Data Images for TGN46 Antibody (9G5X6)

Western Blot: TGN46 Antibody (9G5X6) [NBP3-15817]

Western Blot: TGN46 Antibody (9G5X6) [NBP3-15817]

Western Blot: TGN46 Antibody (9G5X6) [NBP3-15817] - Western blot analysis of extracts of HeLa cells, using TGN46 Rabbit mAb (NBP3-15817) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: TGN46 Antibody (9G5X6) [NBP3-15817]

Immunocytochemistry/ Immunofluorescence: TGN46 Antibody (9G5X6) [NBP3-15817]

Immunocytochemistry/Immunofluorescence: TGN46 Antibody (9G5X6) [NBP3-15817] - Immunofluorescence analysis of U-2 OS cells using TGN46 Rabbit mAb (NBP3-15817) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: TGN46 Antibody (9G5X6) [NBP3-15817]

Immunohistochemistry-Paraffin: TGN46 Antibody (9G5X6) [NBP3-15817]

Immunohistochemistry-Paraffin: TGN46 Antibody (9G5X6) [NBP3-15817] - Immunohistochemistry of paraffin-embedded human liver using TGN46 Rabbit mAb (NBP3-15817) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for TGN46 Antibody (9G5X6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TGN46

The Trans-Golgi network integral membrane protein 2 (TGOLN2) was designated TGN46 based on the predicted molecular mass (46kDa). However, TGN46 is a heavily glycosylated protein, so its molecular weight is often detected at a 110-120kDa. The TGN46 protein is widely expressed. It may be involved in regulating membrane traffic to and from trans-Golgi network, and has been reported as the best available marker for human trans-Golgi network. TGN46 contains a luminal domain, a membrane-spanning domain, and a cytoplasmic domain. The membrane-spanning and cytoplasmic domains contain the retention and retrieval signals, respectively, for localization in the TGN.

Alternate Names

TGN 46, TGN 48, TGN 51, TGN38, TGN-38, TGN38 homolog, TGN46, TGN-46, TGN48, TGN51, TGOLN 2, TGOLN2, trans golgi network 38, trans golgi network 46, Trans Golgi network integral membrane protein 2, Trans Golgi network integral membrane protein 2 [Precursor], Trans Golgi network protein (46 48 51kD isoforms), Trans golgi network protein 2, Trans Golgi network protein TGN51, Trans-Golgi network integral membrane protein 2, Trans-Golgi network protein 2, Trans-Golgi network protein TGN51, TTGN 2, TTGN2

Gene Symbol

TGOLN2

Additional TGN46 Products

Product Documents for TGN46 Antibody (9G5X6)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TGN46 Antibody (9G5X6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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