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TGN46 Antibody [DyLight 650]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49643C

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Bovine, Monkey

Applications

Flow (Intracellular), Flow Cytometry, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

DyLight 650 (Excitation = 652 nm, Emission = 672 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Partial recombinant protein made to an internal region of the human TGN46 protein (within residues 200-350).

Reactivity Notes

Monkey reactivity reported in scientific literature (PMID: 30135710). Bovine reactivity reported in scientific literature (PMID: 30135710).

Localization

Primarily in trans-Golgi network. Cycles between the trans-Golgi network and the cell surface returning via endosomes

Marker

TGN Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TGN46 Antibody [DyLight 650]

Flow Cytometry: TGN46 Antibody [DyLight 650] [NBP1-49643C] - An intracellular stain was performed on HepG2 cells with TGN46 Antibody NBP1-49643C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.

Applications for TGN46 Antibody [DyLight 650]

Application
Recommended Usage

Flow (Intracellular)

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunoblotting

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Knockout Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: TGN46

The TGOLN2 protein may be involved in regulating membrane traffic to and from the trans-Golgi network. There are several isoforms, which are produced by alternative splicing. The upregulation of TGOLN2 has been indicated in association with suicide cases. However, protein analysis has not verified that there is any direct correlation between suicide and TGOLN2 without alcohol influence. It is a highly glycosylated protein.

Alternate Names

TGN 46, TGN 48, TGN 51, TGN38, TGN-38, TGN38 homolog, TGN46, TGN-46, TGN48, TGN51, TGOLN 2, TGOLN2, trans golgi network 38, trans golgi network 46, Trans Golgi network integral membrane protein 2, Trans Golgi network integral membrane protein 2 [Precursor], Trans Golgi network protein (46 48 51kD isoforms), Trans golgi network protein 2, Trans Golgi network protein TGN51, Trans-Golgi network integral membrane protein 2, Trans-Golgi network protein 2, Trans-Golgi network protein TGN51, TTGN 2, TTGN2

Gene Symbol

TGOLN2

Additional TGN46 Products

Product Documents for TGN46 Antibody [DyLight 650]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TGN46 Antibody [DyLight 650]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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