Themis Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90802

Novus Biologicals, part of Bio-Techne
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NBP1-90802
NBP1-90802-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Themis Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EGCESLQPFELPMNFPGLFKIVADKTPYLTMEEITRTIHIGPSRLGHPCFYHQKDIKLENLIIKQGEQIMLNSVEEIDGEIMVSCAVARNHQTHSF

Reactivity Notes

Reactivity reported in scientific literature (PMID: 25535246).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Themis Antibody

Western Blot: Themis Antibody [NBP1-90802]

Western Blot: Themis Antibody [NBP1-90802]

Western Blot: Themis Antibody [NBP1-90802] - Analysis in human cell line Jurkat.
Immunohistochemistry-Paraffin: Themis Antibody [NBP1-90802]

Immunohistochemistry-Paraffin: Themis Antibody [NBP1-90802]

Immunohistochemistry-Paraffin: Themis Antibody [NBP1-90802] - Staining of human thymus shows strong cytoplasmic positivity in cortical cells.

Applications for Themis Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Themis

THEMIS (thymocyte-expressed molecule involved in selection), also called Gasp (Grb2-associating protein), is a 72 kDa, 641 amino acid cytosolic phosphoprotein mainly expressed in late double-negative and double-positive thymocytes. It is involved in thymocyte positive and negative selection through regulation of TCR signaling.

Long Name

Thymocyte-expressed Molecule Involved in Selection

Alternate Names

C6orf190, C6orf207, Gasp, SPOT, TSEPA

Gene Symbol

THEMIS

Additional Themis Products

Product Documents for Themis Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Themis Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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