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Themis Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-29616

Novus Biologicals, part of Bio-Techne
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NBP3-29616

Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Between 591 and 641

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Themis Antibody

Themis Antibody

Immunoprecipitation:Themis AntibodyNBP3-29616] -

Immunoprecipitation:Themis AntibodyNBP3-29616] -Detection of human THEMIS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-THEMIS antibody NBP3-29616 (lot NBP3-29616-1) used for IP at 6 µg per reaction. THEMIS was also immunoprecipitated by rabbit anti-THEMIS antibody BL20901. For blotting immunoprecipitated THEMIS, NBP3-29616 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for Themis Antibody

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate pH 7 -8.

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Themis

THEMIS (thymocyte-expressed molecule involved in selection), also called Gasp (Grb2-associating protein), is a 72 kDa, 641 amino acid cytosolic phosphoprotein mainly expressed in late double-negative and double-positive thymocytes. It is involved in thymocyte positive and negative selection through regulation of TCR signaling.

Long Name

Thymocyte-expressed Molecule Involved in Selection

Alternate Names

C6orf190, C6orf207, Gasp, SPOT, TSEPA

Gene Symbol

THEMIS

Additional Themis Products

Product Documents for Themis Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Themis Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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