Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # THBD/1591

Concentration

0.2 mg/ml

View all Thrombomodulin/BDCA-3 Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa 69-194) of human Thrombomodulin/BDCA-3 protein (exact sequence is proprietary) (Uniprot: P07204)

Localization

Cell Surface & Cytoplasmic

Marker

Endothelial Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54490)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Thrombomodulin/BDCA-3 Antibody (THBD/1591)

Western Blot: Thrombomodulin/BDCA-3 Antibody (THBD/1591) [NBP2-53206]

Western Blot: Thrombomodulin/BDCA-3 Antibody (THBD/1591) [NBP2-53206]

Western Blot: Thrombomodulin/BDCA-3 Antibody (THBD/1591) [NBP2-53206] - Western Blot Analysis (A) Recombinant Protein (B) THP1 Cell lysate Using Thrombomodulin/CD141 Monoclonal Antibody (THBD/1591).
Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody (THBD/1591) [NBP2-53206]

Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody (THBD/1591) [NBP2-53206]

Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody (THBD/1591) [NBP2-53206] - Formalin-fixed, paraffin-embedded human Placenta stained with Thrombomodulin/CD141 Monoclonal Antibody (THBD/1591).
Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody (THBD/1591) [NBP2-53206]

Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody (THBD/1591) [NBP2-53206]

Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody (THBD/1591) [NBP2-53206] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Thrombomodulin Monoclonal/CD141 Antibody (THBD/1591).
View 2 more images

Applications for Thrombomodulin/BDCA-3 Antibody (THBD/1591)

Application
Recommended Usage

ELISA

2-4 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Thrombomodulin/BDCA-3

CD141/Thrombomodulin is an exclusively endothelial cell surface glycoprotein that forms a 1:1 complex with thrombin. Binding of thrombin to this high affinity receptor alters its specificity toward several substrates. The complex activates protein C approximately 1000 times faster than thrombin alone. Activated protein C degrades clotting factors V and VIII; thus, thrombomodulin converts thrombin into a physiologic anticoagulant. Thrombomodulin is also found in the circulatory and urinary systems, the physiologic significance of this is obscure.

Alternate Names

BDCA-3, BDCA3, CD141, Fetomodulin, THBD, THRM

Gene Symbol

THBD

UniProt

P07204 (Mouse)

Additional Thrombomodulin/BDCA-3 Products

Product Documents for Thrombomodulin/BDCA-3 Antibody (THBD/1591)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Thrombomodulin/BDCA-3 Antibody (THBD/1591)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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