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Key Product Details

Species Reactivity

Validated:

Human, Bovine

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation

Label

mFluor Violet 610 SE (Excitation = 421 nm, Emission = 613 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # A65M

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Thrombospondin-1 Antibody (A65M) [mFluor Violet 610 SE]

Immunogen

Extracellular matrix material from cultured bovine corneal endothelial cells

Epitope

Epitope is only present in thrombospondin prepared in low-calcium (0.1 mmol/L) buffers.

Specificity

Highly specific for the low-calcium (0.1 mmol/L) conformation of human TSP1. There is no evidence of cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Thrombospondin-1 Antibody (A65M) [mFluor Violet 610 SE]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Thrombospondin-1

Thrombospondin is a protein from platelet a granules. It is secreted at sites of platelet activation and aggregation and is involved in the differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.

Alternate Names

THBS1, Thrombospondin1, TSP-1

Gene Symbol

THBS1

Additional Thrombospondin-1 Products

Product Documents for Thrombospondin-1 Antibody (A65M) [mFluor Violet 610 SE]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Thrombospondin-1 Antibody (A65M) [mFluor Violet 610 SE]

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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