Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25720
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1194
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free
Immunogen
Recombinant protein encompassing a sequence within the center region of human Thrombospondin-2. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (80%).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free
Immunocytochemistry/Immunofluorescence: Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free [NBP3-25720] -
Immunocytochemistry/Immunofluorescence: Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free [NBP3-25720] - Thrombospondin 2 antibody [HL1194] detects Thrombospondin 2 protein at cytoplasm by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Thrombospondin 2 stained by Thrombospondin 2 antibody [HL1194] (NBP3-25720) diluted at 1:500. Blue: Fluoroshield with DAPI .
ELISA: Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free [NBP3-25720] -
ELISA: Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free [NBP3-25720] - Sandwich ELISA detection of recombinant recombinant HEK293 expressed, full-length human thrombospondin 2 protein using antibodies as below. Capture: Thrombospondin 2 antibody [HL1140] (NBP3-25719) (5 ug/mL) Detection: HRP-conjugated Thrombospondin 2 antibody [HL1194] (NBP3-25720) (1 ug/mL) Please notice that NBP3-25720 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25719. Please contact us for custom HRP-conjugated antibody.
Western Blot: Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free [NBP3-25720] -
Western Blot: Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free [NBP3-25720] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Thrombospondin 2 antibody [HL1194] (NBP3-25720) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Thrombospondin-2
Alternate Names
THBS2, Thrombospondin2, TSP-2
Gene Symbol
THBS2
Additional Thrombospondin-2 Products
Product Documents for Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free
Product Specific Notices for Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...