Thymidylate Synthase Antibody (TYMS/1884)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07144

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2c Kappa Clone # TYMS/1884

Concentration

0.2 mg/ml

View all Thymidylate Synthase Antibodies »

Product Specifications

Immunogen

Recombinant human thymidylate synthase protein fragment (around aa 60-174) (exact sequence is proprietary) (Uniprot: P04818)

Localization

Nuclear & Cytoplasmic

Marker

5-FU Resistance Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2c Kappa

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP3-08882)

Antibody with azide - store at 2 to 8C.

Scientific Data Images for Thymidylate Synthase Antibody (TYMS/1884)

Western Blot: Thymidylate Synthase Antibody (TYMS/1884) [NBP3-07144]

Western Blot: Thymidylate Synthase Antibody (TYMS/1884) [NBP3-07144]

Western Blot: Thymidylate Synthase Antibody (TYMS/1884) [NBP3-07144] - Western Blot Analysis of human HeLa cell lysate using Thymidylate Synthase Mouse Monoclonal Antibody (TYMS/1884).
Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (TYMS/1884) [NBP3-07144]

Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (TYMS/1884) [NBP3-07144]

Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (TYMS/1884) [NBP3-07144] - Formalin-fixed, paraffin-embedded human Tonsil stained with Thymidylate Synthase Mouse Monoclonal Antibody (TYMS/1884).
Flow Cytometry: Thymidylate Synthase Antibody (TYMS/1884) [NBP3-07144]

Flow Cytometry: Thymidylate Synthase Antibody (TYMS/1884) [NBP3-07144]

Flow Cytometry: Thymidylate Synthase Antibody (TYMS/1884) [NBP3-07144] - Flow Cytometric Analysis of PFA-fixed Ramos cells. Thymidylate Synthase Monospecific Mouse Monoclonal Antibody followed by goat anti- Mouse IgG-CF488 (Blue); Isotype Control (Red).
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Applications for Thymidylate Synthase Antibody (TYMS/1884)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Thymidylate Synthase

Thymidylate synthase (TS) is an intracellular enzyme critical for de novo synthesis of DNA. In cancer, expression of TS is often elevated and becomes further elevated as a result of treatment with the most commonly used chemotherapeutic, 5-fluorouracil (5-FU). Resistance or lack of response to 5-FU is attributed to the elevation of TS activity. Overexpression of TS has been demonstrated in many types of tumors including breast, colon, gastric and melanoma and is usually predictive of shortened survival time.

Long Name

Thymidylate synthase

Alternate Names

EC 2.1.1.45, OK/SW-cl.29, TS, TSase, TYMS

Gene Symbol

TYMS

UniProt

P04818 (Human)

Additional Thymidylate Synthase Products

Product Documents for Thymidylate Synthase Antibody (TYMS/1884)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Thymidylate Synthase Antibody (TYMS/1884)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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