Thymosin beta 4 Antibody

Western Blot: Thymosin beta 4 Antibody [H00007114-B01P] -

Western Blot: Thymosin beta 4 Antibody [H00007114-B01P] - No effect of T beta4 on upstream-deleted mutant of NPHP3-promoter (pmt.). (a) Mutant (mt.) NPHP3-promoter plasmids were prepared from wildtype (wt.) promoter. (b–e) HeLa cells were transfected with pCMV-2B or pCMV-T beta4 for 24 h. (b) HeLa cells were co-transfected with wildtype or mutant pEZX-PG02-NPHP3-promoter Gluc plasmid. & Gluc activity in cultured media was measured with luminometer using Gluc substrate. Bar graph indicates the mean of NPHP3-promoter activity. (c) Expression level of T beta4 & NPHP3 transcripts were measured by RT-PCR. (d) The cells were fixed & stained with antibody against Ac-tubulin (red) & DAPI (blue). (e) The ciliated cells in pCMV-2B- (white) or pCMV-T beta4-transfected group (grey) were counted. (f) Mutant (mt.) T beta4-promoter plasmids were prepared from wildtype (wt.) promoter. (g,h) HeLa cells were transfected with pCDNA3.1 or pCDNA6-NPH3 for 24 h. (g) HeLa cells were co-transfected with wildtype or mutant pEZX-PG02-T beta4-promoter Gluc plasmid. & Gluc activity in cultured media was measured with luminometer using Gluc substrate. Bar graph indicates the mean of T beta4-promoter activity. (h) Expression level of T beta4 & NPHP3 transcripts were measured by RT-PCR. Processing (such as changing brightness & contrast) is applied equally to controls across the entire image (c,d & h). Data in a bar graph represent the means ± SEM. **p < 0.01; significantly different from control group. &&p < 0.01; significantly different from wildtype promoter plasmid-transfected group. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31048733), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Thymosin beta 4 Antibody [H00007114-B01P] -

Western Blot: Thymosin beta 4 Antibody [H00007114-B01P] - Effect of T beta4 on primary cilia formation in HeLa cells. (a) HeLa ells were incubated in serum-starved media with 0.1% FBS for 36 h. The cells were fixed & stained with antibody against Ac-tubulin (green) or NPHP3 (red). The representative fluorescence image of primary cilia was shown. (b) Overlay of fluorescence intensity of Ac-tubulin (green) & NPHP3 (red) through the whole length of primary cilia was shown in line graph (Line scan * → ** in a, right). (c,d) Cells were transfected with AccuTarget™ negative control siRNA (NC) or T beta4-siRNA for 24 h. (c) The mRNA (upper) & protein (lower) expression of T beta4 were shown. (d) The cells were incubated in serum-starved media for 36 h, fixed & stained with antibody against Ac-tubulin (green) & DAPI (blue). The ciliated cells in AccuTarget™ negative control siRNA-treated (white) & T beta4-knockdown cells (grey) were counted (n > 500 cells). (e,f) Cells were transfected with pEGFP-2B or pEGFP-T beta4 plasmid for 24 h. (e) The expression of GFP & T beta4-GFP were detected with GFP antibody. (f) The cells were fixed & stained with antibody against Ac-tubulin (red) & DAPI (blue). The ciliated cells in GFP (white) or T beta4-GFP-positive cells (grey) were counted. Processing (such as changing brightness & contrast) is applied equally to controls across the entire image. Data in a bar graph represent the means ± SEM. **p < 0.01; significantly different from control cells. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31048733), licensed under a CC-BY license. Not internally tested by Novus Biologicals.