Thyroid Peroxidase Antibody (2A11)
Novus Biologicals, part of Bio-Techne | Catalog # H00007173-M08
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 2A11
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
TPO (NP_000538.3, 672 a.a. ~ 779 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. WENSHVFTDAQRRELEKHSLSRVICDNTGLTRVPMDAFQVGKFPEDFESCDSITGMNLEAWRETFPQDDKCGFPESVENGDFVHCEESGRRVLVYSCRHGYELQGREQ
Specificity
TPO - thyroid peroxidase (2A11)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Thyroid Peroxidase Antibody (2A11)
![Western Blot: Thyroid Peroxidase Antibody (2A11) [H00007173-M08]](https://resources.bio-techne.com/images/products/Thyroid-Peroxidase-Antibody-2A11-Western-Blot-H00007173-M08-img0004.jpg "Western Blot: Thyroid Peroxidase Antibody (2A11) [H00007173-M08]")
Western Blot: Thyroid Peroxidase Antibody (2A11) [H00007173-M08]
Western Blot: Thyroid Peroxidase Antibody (2A11) [H00007173-M08] - Analysis of TPO expression in transfected 293T cell line by TPO monoclonal antibody (M08), clone 2A11. Lane 1: TPO transfected lysatE (102.9 KDa). Lane 2: Non-transfected lysate.
Sandwich ELISA: Thyroid Peroxidase Antibody (2A11) [H00007173-M08] - Detection limit for recombinant GST tagged TPO is approximately 1ng/ml as a capture antibody.
Applications for Thyroid Peroxidase Antibody (2A11)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Thyroid Peroxidase
Alternate Names
EC 1.11.1, MSA, TDH2A, thyroid microsomal antigen, thyroid peroxidase, thyroperoxidase, TPXEC 1.11.1.8
Entrez Gene IDs
7173 (Human)
Gene Symbol
TPO
UniProt
Additional Thyroid Peroxidase Products
Product Documents for Thyroid Peroxidase Antibody (2A11)
Product Specific Notices for Thyroid Peroxidase Antibody (2A11)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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