Thyroid Peroxidase Antibody (TPO/6417R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07739
Recombinant Monoclonal Antibody
![Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody (TPO/6417R) [NBP3-07739]](https://resources.bio-techne.com/images/products/Thyroid-Peroxidase-Antibody-TPO-6417R-Immunohistochemistry-Paraffin-NBP3-07739-img0002.jpg "Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody (TPO/6417R) [NBP3-07739]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # TPO/6417R
Concentration
0.2 mg/ml
Product Summary for Thyroid Peroxidase Antibody (TPO/6417R)
Immunogen
Recombinant fragment of human Thyroid Peroxidase (around aa 685-804) (Exact sequence is proprietary) (Uniprot: P07202 )
Localization
Cytoplasm, Cell surface
Specificity
Thyroperoxidase (TPO) is a 933 amino acid, type I transmembrane glycoprotein that plays a key role in thyroid gland function and autoimmunity. It is present as a dimer on the apical surface of thyroid follicular cells. TPO functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Malignant thyroid tumors exhibit an anomaly in TPO resulting in lower affinity for anti-TPO. This antibody may aid in the differentiation between benign and malignant thyroid tumors.
Marker
Thyroid Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08762)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Thyroid Peroxidase Antibody (TPO/6417R)
Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody (TPO/6417R) [NBP3-07739]
Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody (TPO/6417R) [NBP3-07739] - Formalin-fixed, paraffin-embedded human thyroid carcinoma stained with Thyroid Peroxidase Recombinant Rabbit Monoclonal Antibody (TPO/6417R).![Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody (TPO/6417R) [NBP3-07739]](https://resources.bio-techne.com/images/products/Thyroid-Peroxidase-Antibody-TPO-6417R-Immunohistochemistry-Paraffin-NBP3-07739-img0001.jpg "Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody (TPO/6417R) [NBP3-07739]")
Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody (TPO/6417R) [NBP3-07739]
Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody (TPO/6417R) [NBP3-07739] - Formalin-fixed, paraffin-embedded human thyroid carcinoma stained with Thyroid Peroxidase Recombinant Rabbit Monoclonal Antibody (TPO/6417R).Applications for Thyroid Peroxidase Antibody (TPO/6417R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Thyroid Peroxidase
Alternate Names
EC 1.11.1, MSA, TDH2A, thyroid microsomal antigen, thyroid peroxidase, thyroperoxidase, TPXEC 1.11.1.8
Gene Symbol
TPO
UniProt
Additional Thyroid Peroxidase Products
Product Documents for Thyroid Peroxidase Antibody (TPO/6417R)
Product Specific Notices for Thyroid Peroxidase Antibody (TPO/6417R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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