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Thyroid Peroxidase Antibody (TPO/7088R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20268

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # TPO/7088R

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Recombinant fragment of human Thyroid Peroxidase (around aa 600-900) (Exact sequence is proprietary)

Localization

Cytoplasm. Cell surface.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Positive Controls: Human thyroid carcinoma.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images for Thyroid Peroxidase Antibody (TPO/7088R)

Thyroid Peroxidase Antibody (TPO/7088R)

Thyroid Peroxidase Antibody (TPO/7088R)

Formalin-fixed, paraffin-embedded human thyroid carcinoma stained with Thyroid Peroxidase antibody (TPO/7088R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
Thyroid Peroxidase Antibody (TPO/7088R)

Thyroid Peroxidase Antibody (TPO/7088R)

Formalin-fixed, paraffin-embedded human thyroid carcinoma stained with Thyroid Peroxidase antibody (TPO/7088R). Inset: PBS instead of primary antibody; secondary only negative control.

Applications for Thyroid Peroxidase Antibody (TPO/7088R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Thyroid Peroxidase

Thyroid Peroxidase encodes a membrane-bound glycoprotein. The encoded protein acts as an enzyme and plays a central role in thyroid gland function. The protein functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Multiple transcript variants encoding distinct isoforms have been identified for this gene. Additional splice variants have been described but their biological natures have not been determined. [provided by RefSeq]

Alternate Names

EC 1.11.1, MSA, TDH2A, thyroid microsomal antigen, thyroid peroxidase, thyroperoxidase, TPXEC 1.11.1.8

Gene Symbol

TPO

Additional Thyroid Peroxidase Products

Product Documents for Thyroid Peroxidase Antibody (TPO/7088R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Thyroid Peroxidase Antibody (TPO/7088R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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