Thyroid Peroxidase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27544

Novus Biologicals, part of Bio-Techne
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NBP2-27544
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Thyroid Peroxidase Antibodies »

Product Specifications

Immunogen

Peptide with sequence ELRCGKHQAVGTSPQ corresponding to C-Terminus according to NP_000538.3, NP_783650.1, NP_783652.1, NP_783653.1.

Specificity

This antibody is expected to recognize all reported isoforms (NP_000538.3; NP_783650.1; NP_783652.1; NP_783653.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Thyroid Peroxidase Antibody

Western Blot: Thyroid Peroxidase Antibody [NBP2-27544]

Western Blot: Thyroid Peroxidase Antibody [NBP2-27544]

Western Blot: Thyroid Peroxidase Antibody [NBP2-27544] - (0.1 ug/ml) Analysis of Thyroid Gland lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody [NBP2-27544]

Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody [NBP2-27544]

Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody [NBP2-27544] - Staining of Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Thyroid Peroxidase Antibody

Application
Recommended Usage

Immunohistochemistry

5 - 10 ug/ml

Immunohistochemistry-Paraffin

5-10 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 110 kDa band observed in human thyroid gland lysates (calculated MW of 103 kDa band according to NP_000538.3). IHC-P: Human thyroid gland shows textured staining in the cytoplasm of epithelial cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Thyroid Peroxidase

Thyroid Peroxidase encodes a membrane-bound glycoprotein. The encoded protein acts as an enzyme and plays a central role in thyroid gland function. The protein functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Multiple transcript variants encoding distinct isoforms have been identified for this gene. Additional splice variants have been described but their biological natures have not been determined. [provided by RefSeq]

Alternate Names

EC 1.11.1, MSA, TDH2A, thyroid microsomal antigen, thyroid peroxidase, thyroperoxidase, TPXEC 1.11.1.8

Gene Symbol

TPO

UniProt

NP_000538.3 (Human)

Additional Thyroid Peroxidase Products

Product Documents for Thyroid Peroxidase Antibody

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Certificate of Analysis

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Product Specific Notices for Thyroid Peroxidase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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