TIF1 alpha [p Ser1043] Antibody (7N5L6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33568

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 7N5L6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TIF1 alpha Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around S1043 of mouse TIF1 alpha (NP_659542.3).

Sequence:
LKSTE

Modification

p Ser 1043

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

117 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TIF1 alpha [p Ser1043] Antibody (7N5L6)

TIF1 alpha [p Ser1043] Antibody (7N5L6)

Western Blot: TIF1 alpha [p Ser1043] Antibody (7N5L6) [NBP3-33568] -

Western Blot: TIF1 alpha [p Ser1043] Antibody (7N5L6) [NBP3-33568] - Western blot analysis of lysates from HeLa cells, using TIF1 alpha Rabbit mAb at1:1000 dilution. Hela cells were treated by TGF-beta (10 ng/ml) at 37C for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 180s.

Applications for TIF1 alpha [p Ser1043] Antibody (7N5L6)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TIF1 alpha

TIF1 alpha is encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Long Name

Transcription intermediary factor 1-alpha

Alternate Names

RNF82, TIF1, TIF1-alpha, TIF1A, TRIM24

Additional TIF1 alpha Products

Product Documents for TIF1 alpha [p Ser1043] Antibody (7N5L6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TIF1 alpha [p Ser1043] Antibody (7N5L6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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