TIF1 alpha Antibody (JE64-31)

Western Blot: TIF1 alpha Antibody (JE64-31) [NBP3-33052]

Western Blot: TIF1 alpha Antibody (JE64-31) [NBP3-33052] - Western blot analysis of TIF1 alpha on different lysates with Rabbit anti-TIF1 alpha antibody at 1/500 dilution.

Lane 1: HepG2 cell lysate
Lane 2: HeLa cell lysate

Lysates/proteins at 10 ug/Lane.

Predicted band size: 117 kDa
Observed band size: 130 kDa

Exposure time: 2 minutes;

6 % SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody TRIM24 at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: TIF1 alpha Antibody (JE64-31) [NBP3-33052]

Immunohistochemistry: TIF1 alpha Antibody (JE64-31) [NBP3-33052] - Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue with Rabbit anti-TIF1 alpha antibody at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody TRIM24 at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.