TIF1 alpha Antibody (PCRP-TRIM24-1B12)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07464

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # PCRP-TRIM24-1B12

Concentration

0.2 mg/ml

View all TIF1 alpha Antibodies »

Product Summary for TIF1 alpha Antibody (PCRP-TRIM24-1B12)

Immunogen

Recombinant full-length human TIF1 alpha protein (Uniprot: O15164)

Localization

Nucleus, Cytoplasm. Colocalizes with sites of active transcription.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

117 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08786)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for TIF1 alpha Antibody (PCRP-TRIM24-1B12)

Immunocytochemistry/ Immunofluorescence: TIF1 alpha Antibody (PCRP-TRIM24-1B12) [NBP3-07464]

Immunocytochemistry/ Immunofluorescence: TIF1 alpha Antibody (PCRP-TRIM24-1B12) [NBP3-07464]

Immunocytochemistry/Immunofluorescence: TIF1 alpha Antibody (PCRP-TRIM24-1B12) [NBP3-07464] - Immunofluorescent Analysis of PFA-fixed MCF7 cells. TIF1 alpha Mouse Monoclonal Antibody (PCRP-TRIM24-1B12) followed by IgG-CF488 (green), counterstained with phalloidin.
Flow Cytometry: TIF1 alpha Antibody (PCRP-TRIM24-1B12) [NBP3-07464]

Flow Cytometry: TIF1 alpha Antibody (PCRP-TRIM24-1B12) [NBP3-07464]

Flow Cytometry: TIF1 alpha Antibody (PCRP-TRIM24-1B12) [NBP3-07464] - Flow Cytometric Analysis of PFA-fixed HeLa cells. TIF1 alpha Mouse Monoclonal Antibody (PCRP-TRIM24-1B12) followed by goat anti- Mouse IgG-CF488 (blue); isotype control (red).
Protein Array: TIF1 alpha Antibody (PCRP-TRIM24-1B12) [NBP3-07464]

Protein Array: TIF1 alpha Antibody (PCRP-TRIM24-1B12) [NBP3-07464]

Protein Array: TIF1 alpha Antibody (PCRP-TRIM24-1B12) [NBP3-07464] - Analysis of Protein Array containing more than 19,000 full-length human proteins using TIF1 alpha Mouse Monoclonal Antibody (PCRP-TRIM24-1B12).

Applications for TIF1 alpha Antibody (PCRP-TRIM24-1B12)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: TIF1 alpha

TIF1 alpha is encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Long Name

Transcription intermediary factor 1-alpha

Alternate Names

RNF82, TIF1, TIF1-alpha, TIF1A, TRIM24

Gene Symbol

TRIM24

UniProt

O15164 (Human)

Additional TIF1 alpha Products

Product Documents for TIF1 alpha Antibody (PCRP-TRIM24-1B12)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TIF1 alpha Antibody (PCRP-TRIM24-1B12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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