Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all TIF1 alpha Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1,000 and the C-terminus (residue 1050) of human Transcriptional Intermediary Factor 1 Alpha (NP_056989.2).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

117 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TIF1 alpha Antibody

Western Blot: TIF1 alpha Antibody [NB100-2597]

Western Blot: TIF1 alpha Antibody [NB100-2597]

Western Blot: TIF1 alpha Antibody [NB100-2597] - Detection of Human TIF1 alpha/TRIM24 on HeLa whole cell lysate (5, 15, and 50 microgram) and 293T cell lysate (50 microgram) using NB100-2597. TIF1 alpha/TRIM24 was also immunoprecipitated by rabbit anti-TIF1 alpha/TRIM24 antibody NB100-2596.

Applications for TIF1 alpha Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Use in Immunohistochemistry-paraffin and Immunohistochemistry-Frozen reported in scientific literature (PMID: 23006314). Use in ICC/IF was reported in scientific literature (PMID: 23006314).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TIF1 alpha

The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Long Name

Transcription intermediary factor 1-alpha

Alternate Names

RNF82, TIF1, TIF1-alpha, TIF1A, TRIM24

Entrez Gene IDs

8805 (Human); 21848 (Mouse)

Gene Symbol

TRIM24

UniProt

O15164 (Human)

Additional TIF1 alpha Products

Product Documents for TIF1 alpha Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TIF1 alpha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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