Tight Junction Protein 1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85046
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGR
Reactivity Notes
Rat reactivity reported in scientific literature (PMID: 31174067).
Marker
Intercellular Junctions/Tight Junction Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Tight Junction Protein 1 Antibody
Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85046]
Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85046] - Staining in human cerebral cortex and skeletal muscle tissues using anti-TJP1 antibody. Corresponding TJP1 RNA-seq data are presented for the same tissues.Western Blot: Tight Junction Protein 1 Antibody [NBP1-85046]
Western Blot: Tight Junction Protein 1 Antibody [NBP1-85046] - Analysis of Tight Junction Protein 1 in RIPA whole cell lysates of mouse primary choroid plexus epithelial cells using anti-Tight Junction Protein 1 antibody. Image from verified customer review.Immunocytochemistry/ Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85046]
Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85046] - Staining of human cell line U-2 OS shows localization to cytosol & cell junctions. Antibody staining is shown in green.Applications for Tight Junction Protein 1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25 - 2 ug/mL
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Frozen
validated from a verified customer review.
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
validated from a verified customer review.
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4.5 using NBP1-85046 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Tight Junction Protein 1
Alternate Names
DKFZp686M05161, MGC133289, Tight junction protein 1, tight junction protein 1 (zona occludens 1), tight junction protein ZO-1, TJP1, ZO1, ZO-1, zona occludens 1, Zona occludens protein 1, zonula occludens 1 protein, Zonula occludens protein 1
Gene Symbol
TJP1
Additional Tight Junction Protein 1 Products
Product Documents for Tight Junction Protein 1 Antibody
Product Specific Notices for Tight Junction Protein 1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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