TIM-4 Antibody (3A1) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11990
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: TIM-4 Antibody (3A1) - Chimeric - Azide and BSA Free [NBP3-11990]](https://resources.bio-techne.com/images/products/TIM-4-Antibody-3A1-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-11990-img0001.jpg "Immunocytochemistry/ Immunofluorescence: TIM-4 Antibody (3A1) - Chimeric - Azide and BSA Free [NBP3-11990]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Block/Neutralize, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # 3A1
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was raised by immunising female Lewis rats (Harlan Sprague-Dawley) with TIM-4:Ig fusion protein in complete Freund's adjuvant.
Specificity
This antibody is specific for murine TIM-4, which unlike other TIM family members, is not expressed on T lymphocytes but rather found on antigen presenting cells such as lymphoid and myeloid DC's and macrophages. TIM-4 has been shown to interact with TIM-1 thereby suggesting a role of TIM-4 in the balance of Th1/Th2 regulation. Additionally TIM-4 has been shown to be a receptor for phosphatidylserine and may play a role in engulfment of apoptotic cells.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for TIM-4 Antibody (3A1) - Chimeric - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: TIM-4 Antibody (3A1) - Chimeric - Azide and BSA Free [NBP3-11990]
Immunocytochemistry/Immunofluorescence: TIM-4 Antibody (3A1) - Chimeric [NBP3-11990] - Immunofluorescence analysis of paraformaldehyde fixed RAW 264.7 cells stained with the chimeric rabbit IgG version of 3A1 (NBP3-11990) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11990, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (NBP2-52638) followed by Alexa Fluor(R) 488 secondary antibody.Applications for TIM-4 Antibody (3A1) - Chimeric - Azide and BSA Free
Application
Recommended Usage
Block/Neutralize
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: TIM-4
Long Name
T Cell Immunoglobulin Mucin-4
Alternate Names
Smuckler, TIM4, TIMD4
Gene Symbol
TIMD4
Additional TIM-4 Products
Product Documents for TIM-4 Antibody (3A1) - Chimeric - Azide and BSA Free
Product Specific Notices for TIM-4 Antibody (3A1) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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