TIMM23 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33018
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic peptide within human TIMM23 aa 150-209. (Uniprot: O14925)
Localization
Mitochondrion inner membrane.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for TIMM23 Antibody
Western Blot: TIMM23 Antibody [NBP3-33018]
Western Blot: TIMM23 Antibody [NBP3-33018] - Western blot analysis of TIMM23 on different lysates with Rabbit anti-TIMM23 antibody (NBP3-33018) at 1/500 dilution.Lane 1: NIH/3T3 cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: RAW264.7 cell lysate
Lysates/proteins at 10 ug/Lane.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33018) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
Immunohistochemistry: TIMM23 Antibody [NBP3-33018]
Immunohistochemistry: TIMM23 Antibody [NBP3-33018] - Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-TIMM23 antibody (NBP3-33018) at 1/800 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33018) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Applications for TIMM23 Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:800
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TIMM23
Alternate Names
bA592B15.7, MGC22767, PRO1197, RP11-592B15.7, TIM23
Gene Symbol
TIMM23
Additional TIMM23 Products
Product Documents for TIMM23 Antibody
Product Specific Notices for TIMM23 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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