TIMM29 Antibody

Western Blot: TIMM29 Antibody [NBP3-12622]

Western Blot: TIMM29 Antibody [NBP3-12622] - Lane A: RT4 Whole Cell LysateLane B: U-251 MG Whole Cell LysateLane C: NIH/3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:29 kDa

Immunocytochemistry/ Immunofluorescence: TIMM29 Antibody [NBP3-12622]

Immunocytochemistry/Immunofluorescence: TIMM29 Antibody [NBP3-12622] - Staining of C19orf52 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human C19orf52 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus and Cytoplasm.

Immunoprecipitation: TIMM29 Antibody [NBP3-12622]

Immunoprecipitation: TIMM29 Antibody [NBP3-12622] - Lane A:0.5 mg RT-4 Whole Cell Lysate4 uL anti-C19orf52 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-C19orf52 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 29 kDaObserved band size :29 kDa