TIMM44 Antibody (JE65-55)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33020
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE65-55
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within human TIMM44 aa 1-280/452. (Uniprot: O43615)
Localization
Mitochondrion inner membrane, Mitochondrion matrix.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for TIMM44 Antibody (JE65-55)
Western Blot: TIMM44 Antibody (JE65-55) [NBP3-33020]
Western Blot: TIMM44 Antibody (JE65-55) [NBP3-33020] - Western blot analysis of TIMM44 on different lysates with Rabbit anti-TIMM44 antibody (NBP3-33020) at 1/500 dilution.Lane 1: PC-3 cell lysate, 10 ug/Lane
Lane 2: 293 cell lysate, 10 ug/Lane
Lane 3: Rat stomach tissue lysate, 20 ug/Lane
Predicted band size: 51 kDa
Observed band size: 40 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33020) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
Applications for TIMM44 Antibody (JE65-55)
Application
Recommended Usage
Western Blot
1:500
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TIMM44
Alternate Names
MIMT44, mitochondrial import inner membrane translocase subunit TIM44, mitochondrial inner membrane translocase, TIM44DKFZp686H05241, translocase of inner mitochondrial membrane 44 homolog (yeast)
Gene Symbol
TIMM44
Additional TIMM44 Products
Product Documents for TIMM44 Antibody (JE65-55)
Product Specific Notices for TIMM44 Antibody (JE65-55)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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