TIMP-1 Antibody (F31 P2 A5)
Novus Biologicals, part of Bio-Techne | Catalog # NB100-74551
![Immunocytochemistry/ Immunofluorescence: TIMP-1 Antibody (F31 P2 A5) [NB100-74551]](https://resources.bio-techne.com/images/products/TIMP-1-Antibody-F31-P2-A5-Immunocytochemistry-Immunofluorescence-NB100-74551-img0004.jpg "Immunocytochemistry/ Immunofluorescence: TIMP-1 Antibody (F31 P2 A5) [NB100-74551]")
![Immunohistochemistry-Paraffin: TIMP-1 Antibody (F31 P2 A5) [NB100-74551]](https://resources.bio-techne.com/images/products/TIMP-1-Antibody-F31-P2-A5-Immunohistochemistry-Paraffin-NB100-74551-img0007.jpg "Immunohistochemistry-Paraffin: TIMP-1 Antibody (F31 P2 A5) [NB100-74551]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Monkey
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # F31 P2 A5
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide corresponding to residues F(72) Q A L G D A A D I R(82) of mouse TIMP-1.
Reactivity Notes
Rhesus monkey. Mouse reactivity reported in scientific literature (PMID: 19188864). Rat reactivity reported in scientific literature (PMID: 28275350).
Localization
Secreted
Specificity
TIMP-1 (F31 P2 A5)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for TIMP-1 Antibody (F31 P2 A5)
![Western Blot: TIMP-1 Antibody (F31 P2 A5) [NB100-74551]](https://resources.bio-techne.com/images/products/TIMP-1-Antibody-F31-P2-A5-Western-Blot-NB100-74551-img0009.jpg "Western Blot: TIMP-1 Antibody (F31 P2 A5) [NB100-74551]")
Western Blot: TIMP-1 Antibody (F31 P2 A5) [NB100-74551]
Western Blot: TIMP-1 Antibody (F31 P2 A5) [NB100-74551] - Analysis was performed on whole cell extracts (30 ug lysate) of A431 (Lane 1), HeLa (Lane 2), Jurkat (Lane 3), HT29 (Lane 4) and COS-7 (Lane 5). The blots were probed with Anti-TIMP1 Mouse .![Immunocytochemistry/ Immunofluorescence: TIMP-1 Antibody (F31 P2 A5) [NB100-74551]](https://resources.bio-techne.com/images/products/TIMP-1-Antibody-F31-P2-A5-Immunocytochemistry-Immunofluorescence-NB100-74551-img0005.jpg "Immunocytochemistry/ Immunofluorescence: TIMP-1 Antibody (F31 P2 A5) [NB100-74551]")
Immunocytochemistry/ Immunofluorescence: TIMP-1 Antibody (F31 P2 A5) [NB100-74551]
Immunocytochemistry/Immunofluorescence: TIMP-1 Antibody (F31 P2 A5) [NB100-74551] - Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a TIMP1 Monclonal Antibody (F31 P2 A5) at a dilution of 1:20 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI.![Immunohistochemistry-Paraffin: TIMP-1 Antibody (F31 P2 A5) [NB100-74551]](https://resources.bio-techne.com/images/products/TIMP-1-Antibody-F31-P2-A5-Immunohistochemistry-Paraffin-NB100-74551-img0006.jpg "Immunohistochemistry-Paraffin: TIMP-1 Antibody (F31 P2 A5) [NB100-74551]")
Immunohistochemistry-Paraffin: TIMP-1 Antibody (F31 P2 A5) [NB100-74551]
Immunohistochemistry-Paraffin: TIMP-1 Antibody (F31 P2 A5) [NB100-74551] - Normal biopsies of deparaffinized Human prostate tissue at a dilution of 1:20.Applications for TIMP-1 Antibody (F31 P2 A5)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1 - 2 ug/ml
Immunohistochemistry
1:20
Immunohistochemistry-Paraffin
1:10 - 1:100
Western Blot
1:250 - 1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: TIMP-1
Long Name
Tissue Inhibitors of Metalloproteinases 1
Alternate Names
TIMP1
Entrez Gene IDs
7076 (Human)
Gene Symbol
TIMP1
Additional TIMP-1 Products
Product Documents for TIMP-1 Antibody (F31 P2 A5)
Product Specific Notices for TIMP-1 Antibody (F31 P2 A5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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